Comparative analysis of lignin peroxidase and manganese peroxidase activity on coniferous and deciduous wood using ToF-SIMS
| Autor: | Jacqueline MacDonald, Mamdouh Abou-Zaid, Robyn E. Goacher, Emma R. Master |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Spectrometry Mass Secondary Ion macromolecular substances Lignin complex mixtures 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Manganese peroxidase Organic chemistry Wood fibre biology fungi Fungi technology industry and agriculture food and beverages Manganese peroxidase activity General Medicine Lignin peroxidase Wood 030104 developmental biology Deciduous Peroxidases chemistry biology.protein Oxidative cleavage 010606 plant biology & botany Biotechnology Peroxidase |
| Zdroj: | Applied Microbiology and Biotechnology. 100:8013-8020 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-016-7560-2 |
| Popis: | White-rot fungi are distinguished by their ability to efficiently degrade lignin via lignin-modifying type II peroxidases, including manganese peroxidase (MnP) and lignin peroxidase (LiP). In the present study, time-of flight secondary ion mass spectrometry (ToF-SIMS) was used to evaluate lignin modification in three coniferous and three deciduous wood preparations following treatment with commercial preparations of LiP and MnP from two different white-rot fungi. Percent modification of lignin was calculated as a loss of intact methoxylated lignin over nonfunctionalized aromatic rings, which is consistent with oxidative cleavage of methoxy moieties within the lignin structure. Exposure to MnP resulted in greater modification of lignin in coniferous compared to deciduous wood (28 vs. 18 % modification of lignin); and greater modification of G-lignin compared to S-lignin within the deciduous wood samples (21 vs. 12 %). In contrast, exposure to LiP resulted in similar percent modification of lignin in all wood samples (21 vs 22 %), and of G- and S-lignin within the deciduous wood (22 vs. 23 %). These findings suggest that the selected MnP and LiP may particularly benefit delignification of coniferous and deciduous wood, respectively. Moreover, the current analysis further demonstrates the utility of ToF-SIMS for characterizing enzymatic modification of lignin in wood fibre along with potential advantages over UV and HPCL-MS detection of solubilized delignification products. |
| Databáze: | OpenAIRE |
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