Structural characterization of anticoagulant and antithrombotic polysaccharides isolated from Caesalpinia ferrea stem barks
| Autor: | Ana Maria Sampaio Assreuy, Francisco F. Bezerra, Paulo A.S. Mourão, Arcelina Pacheco Cunha, Maria Gonçalves Pereira, Diego Freitas de Araujo, Nágila M.P.S. Ricardo, Juliana C. Madeira |
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| Rok vydání: | 2021 |
| Předmět: |
Arabinose
Platelet Aggregation Rhamnose 02 engineering and technology Uronic acid Polysaccharide Biochemistry Gel permeation chromatography 03 medical and health sciences chemistry.chemical_compound Fibrinolytic Agents Polysaccharides Structural Biology Animals Humans Rats Wistar Blood Coagulation Molecular Biology Polyacrylamide gel electrophoresis 030304 developmental biology Venous Thrombosis chemistry.chemical_classification 0303 health sciences Caesalpinia Chromatography Plant Extracts Anticoagulants Polyphenols General Medicine 021001 nanoscience & nanotechnology Rats chemistry Polyphenol Plant Bark Partial Thromboplastin Time Arachidonic acid 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 175:147-155 |
| ISSN: | 0141-8130 |
| Popis: | This study aimed to isolate, characterize chemical-structurally and evaluate the effects of polysaccharides from Caesalpinia (Libidibia) ferrea stem barks in the haemostatic system. The deproteinated-polysaccharide extract (PE-Cf) after being fractionated by ion exchange chromatography-DEAE-cellulose resulted in three fractions (FI, FII, FIII) containing total carbohydrates (14.3–38%), including uronic acid (5–16%), and polyphenols (0.94–1.7 mg/g GAE). The polysaccharide fractions presented polydisperse profile in polyacrylamide gel electrophoresis (detected by Stains-All) and molecular masses (9.5 × 104 Da–1.5 × 105 Da) identified by gel permeation chromatography. FT-IR showed absorption bands (1630 cm−1, 1396–1331 cm−1), indicative of uronic acid, and a band at 1071 cm−1, typical of COO− groups of galacturonic acid. The NMR spectra of C. ferrea polysaccharides revealed a central core composed mainly by 5-linked α-Araf and minority components as α-Rhap and α-GalAp. UV spectra of fractions revealed discrete shoulders at 269–275 nm, characteristic of polyphenolic compounds. In vitro, polysaccharides inhibited the intrinsic and/or common coagulation pathway (aPTT test) (2.0–3.7 fold) and the platelet aggregation induced by 3 μM adenosine diphosphate (25–48%) and 5 μg/mL collagen (24%), but not that induced by arachidonic acid. In vivo, the polysaccharides inhibited (36–69%) venous thrombosis induced by hypercoagulability and stasis, showing discrete hemorrhagic effect. In conclusion, the polysaccharides of C. ferrea barks, containing arabinose, galactose, rhamnose and uronic acid, possess anticoagulant, antiplatelet and antithrombotic properties of low hemorrhagic risk, suggesting potential applicability in thromboembolic disorders. |
| Databáze: | OpenAIRE |
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