The effects of hyperglycemia on ischemic cell change and reactive neuronal change in neonatal rat brain following transient forebrain ischemia
| Autor: | Tohru Sugimoto, Hideyuki Nagai, Tadaki Ohmae, Hiroshi Yoshioka, Taro Tanaka |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Cell Ischemia Carbohydrate metabolism Statistics Nonparametric Brain ischemia Prosencephalon Developmental Neuroscience Internal medicine Occlusion Mole medicine Animals Lactic Acid Rats Wistar skin and connective tissue diseases Neurons Neonatal rat Neocortex business.industry General Medicine medicine.disease Rats Survival Rate medicine.anatomical_structure Endocrinology Glucose Animals Newborn Ischemic Attack Transient Anesthesia Hyperglycemia Pediatrics Perinatology and Child Health sense organs Neurology (clinical) business |
| Zdroj: | Braindevelopment. 30(2) |
| ISSN: | 0387-7604 |
| Popis: | To examine the effects of hyperglycemia on a transient ischemia in the neonatal brain, neuropathological and biochemical evaluations were performed. In 10-day-old rats, brain ischemia was induced by permanent occlusion of the right external and internal carotid and subclavian arteries and the clamping of the left external and internal carotid arteries for 2h. The peritoneal injection of a 50% glucose solution (0.10 ml/15 g weight) 5 min before the induction of brain ischemia increased the plasma glucose concentration to 20-25 mmol/l during ischemia. It preserved brain tissue glucose levels at 1h of ischemia in the glucose-treated group, while tissue glucose was exhausted in the saline-injected group. Tissue lactate concentrations increased slightly at the end of the ischemic insult (6.7 mmol/kg) in the saline-injected group and remarkably (18.7 mmol/kg) in the glucose-treated group. Two distinct forms of ischemic neuronal change were found in this study: ischemic cell change and reactive neuronal change. A quantitative neuropathological assessment indicated that hyperglycemia significantly reduced the volume of ischemic cell change in the neocortex from 85% to 33%, but not that of reactive neuronal change (from 5.5% to 2.4%). These results indicated that hyperglycemia attenuated ischemic cell change, but not reactive neuronal change, in the neonatal rat brain and suggested that it reduced ischemic cell change probably because of reserved brain glucose. |
| Databáze: | OpenAIRE |
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