Δ5 desaturation of fatty acids in L-M cells
| Autor: | Merle L. Blank, Ten-ching Lee, Fred Snyder, Edward N. Lambremont |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Fatty Acid Desaturases
chemistry.chemical_classification Ozonolysis Double bond Stereochemistry Linoleic acid Biophysics Fatty acid Cell Biology Biochemistry Cell Line Amino acid chemistry.chemical_compound 8 11 14-Eicosatrienoic Acid Eicosadienoic Acid Enzyme Linoleic Acids chemistry Choline Molecular Biology |
| Zdroj: | Biochemical and Biophysical Research Communications. 80:813-818 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(78)91317-7 |
| Popis: | L-M cells grown in a lipid-free medium containing /sup 14/C-labeled 9, 12-linoleic acid incorporated most of this acid into glycerolipids as linoleic acid. Only a small amount (3%) was elongated to eicosadienoic acid. No ..delta..6 desaturation occurred. When the cells were incubated with /sup 14/-C-labeled 8, 11, 14-eicosatrienoic acid, 22% of the activity was found in 5, 8, 11, 14-eicosatetraenoic acid. Treatment of the cells for 24 hr with N-isopropylethanolamine, a choline analog, depressed this desaturation reaction to about 60% of control values. The identity of the tetraene product was established by two different chromatographic analyses of the fatty acid methyl esters. Location of the double bond at position C-5 was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas-liquid chromatography. These results prove that L-M cells have a ..delta..5 desaturase and an elongation enzyme converting 18 : 2 to 20 : 2, but lack a ..delta..6 desaturase. |
| Databáze: | OpenAIRE |
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