Production of biovanillin by one-step biotransformation using fungus Pycnoporous cinnabarinus
| Autor: | Mahesh V. Bule, Uday S. Annapure, Ashwini Tilay |
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| Rok vydání: | 2010 |
| Předmět: |
Chromatography
Vanillin Basidiomycota chemistry.chemical_element General Chemistry One-factor-at-a-time method Nitrogen Corn steep liquor Ferulic acid chemistry.chemical_compound Industrial Microbiology chemistry Biotransformation Benzaldehydes Organic chemistry Composition (visual arts) Ammonium chloride General Agricultural and Biological Sciences |
| Zdroj: | Journal of agricultural and food chemistry. 58(7) |
| ISSN: | 1520-5118 |
| Popis: | The current study proposes a one-step biotransformation process for vanillin production from ferulic acid using the wild fungal strain Pycnoporous cinnabarinus belonging to the family Basidiomycete. Improvement of biotransformation conditions was performed in two steps; initially a one factor at a time method was used to investigate effects of medium composition variables (i.e., carbon, nitrogen) and environmental factors such as pH on vanillin production. Subsequently, concentrations of medium components were optimized using an orthogonal matrix method. After primary screening, glucose as carbon source and corn steep liquor and ammonium chloride as organic and inorganic nitrogen source, respectively, supported maximum biotransformation of ferulic acid to vanillin. Under statistically optimum conditions vanillin production from ferulic acid by P. cinnabarinus was 126 mg/L with a molar yield of 54%. The overall molar yield of vanillin production increased by 4 times. |
| Databáze: | OpenAIRE |
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