Interactions of the HIV-1 reverse transcriptase 'AZT-resistant' mutant with substrates and AZT-TP

Autor:	Sergey N. Kochetkov, D K Pokholok, Vladimir O. Rechinsky, S. O. Gudima, Dmitry S. Yesipov, V. N. Dobrynin
Rok vydání:	1993
Předmět:	viruses Mutant Biophysics Mutagenesis (molecular biology technique) Biology medicine.disease_cause Biochemistry Antiviral Agents Substrate Specificity Zidovudine chemistry.chemical_compound Structural Biology immune system diseases Genetics medicine Reverse transcriptase Thymine Nucleotides heterocyclic compounds ‘AZT-resistant’ mutation Molecular Biology Mutation DNA synthesis Wild type virus diseases Drug Resistance Microbial RNA-Directed DNA Polymerase Cell Biology biochemical phenomena metabolism and nutrition Virology Molecular biology HIV Reverse Transcriptase Recombinant Proteins chemistry DNA Viral HIV-1 Mutagenesis Site-Directed Reverse Transcriptase Inhibitors DNA medicine.drug Dideoxynucleotides
Zdroj:	FEBS letters. 325(3)
ISSN:	0014-5793
Popis:	To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. ‘AZT-resistant’ mutations in RT were shown to be associated with the reduced capability of AZT-TP to block the DNA- but not RNA-directed DNA synthesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::299073cd36828a29ba9d77d19c6f5c7e https://pubmed.ncbi.nlm.nih.gov/7686510 Zobrazit plný text záznamu Plný text