The endocrine disruptor mono-(2-ethylhexyl)phthalate promotes adipocyte differentiation and induces obesity in mice

Autor: Xu Ma, Chan-Juan Hao, Hong-Fei Xia, Xuejia Cheng
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Blood Glucose
Male
obesity
PVC
poly(vinyl chloride)
PND
postnatal day
lcsh:Life
lcsh:QR1-502
Adipose tissue
Endocrine Disruptors
Biochemistry
DMEM
Dulbecco’s modified Eagle’s medium
lcsh:Microbiology
Srebf1
sterol-regulatory-element-binding factor 1
Mice
chemistry.chemical_compound
Pregnancy
Adipocyte
Adipocytes
Receptor
peroxisome proliferator-activated receptor γ
Phthalate
FAS
fatty acid synthase
Lipids
LPL
lipoprotein lipase
b.w.
body weight
Endocrine disruptor
C/EBPα
CCAAT/enhancer-binding protein α
Adipogenesis
Prenatal Exposure Delayed Effects
Female
PPAR
peroxisome-proliferator-activated receptor
aP2
adipocyte-specific fatty acid binding protein
medicine.medical_specialty
TAG
triacylglycerol
Offspring
MEHP
mono(2-ethylhexyl)phthalate
GPDH
glycerol-3-phosphate dehydrogenase
Biophysics
NRIFP
National Research Institute for Family Planning
Biology
perinatal exposure
S2
S4
adipogenesis
EDC
endocrine disrupting chemical
Sex Factors
3T3-L1 Cells
Diethylhexyl Phthalate
Internal medicine
medicine
Animals
DEHP
di-(2-ethylhexyl) phthalate
Molecular Biology
Original Paper
Correction
Cell Biology
BPA
bisphenol A
Mice
Inbred C57BL
lcsh:QH501-531
Endocrinology
chemistry
mono(2-ethylhexyl) phthalate (MEHP)
Obesogen
Zdroj: Bioscience Reports, Vol 32, Iss 6 (2012)
Bioscience Reports
ISSN: 1573-4935
0144-8463
Popis: The environmental obesogen hypothesis proposes that exposure to endocrine disruptors during developmental ‘window’ contributes to adipogenesis and the development of obesity. MEHP [mono-(2-ethylhexyl) phthalate], a metabolite of the widespread plasticizer DEHP [di-(2-ethylhexyl) phthalate], has been found in exposed organisms and identified as a selective PPARγ (peroxisome-proliferator-activated receptor γ) modulator. However, implication of MEHP on adipose tissue development has been poorly investigated. In the present study, we show the dose-dependent effects of MEHP on adipocyte differentiation and GPDH (glycerol-3-phosphate dehydrogenase) activity in the murine 3T3-L1 cell model. MEHP induced the expression of PPARγ as well as its target genes required for adipogenesis in vitro. Moreover, MEHP perturbed key regulators of adipogenesis and lipogenic pathway in vivo. In utero exposure to a low dose of MEHP significantly increased b.w. (body weight) and fat pad weight in male offspring at PND (postnatal day) 60. In addition, serum cholesterol, TAG (triacylglycerol) and glucose levels were also significantly elevated. These results suggest that perinatal exposure to MEHP may be expected to increase the incidence of obesity in a sex-dependent manner and can act as a potential chemical stressor for obesity and obesity-related disorders.
Databáze: OpenAIRE
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