Analysis of macrophages and neutrophils infiltrating murine mammary carcinoma sites within hours of tumor delivery
| Autor: | Silvia Chilel Martin, Catherine M Newsom-Stewart, Robert A. Kurt, Matthew DeBenedetto, Amanda Lauricella, Alison Baranovic, Matthew Adusei, Jennifer Schwartz, Chenyu Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Neutrophils Cytokine profile Immunology Oxidative phosphorylation Biology Oxidative Phosphorylation Mammary carcinoma 03 medical and health sciences Mice 0302 clinical medicine Immune system Phagocytosis Transforming Growth Factor beta Cell Line Tumor Animals Atp production chemistry.chemical_classification Reactive oxygen species Mice Inbred BALB C Tumor Necrosis Factor-alpha Macrophages Mammary Neoplasms Experimental Phenotype 030104 developmental biology chemistry Neutrophil Infiltration Cancer research Female Reactive Oxygen Species Glycolysis Metabolic profile 030215 immunology |
| Zdroj: | Cellular immunology. 346 |
| ISSN: | 1090-2163 |
| Popis: | Here we used three different murine mammary carcinomas to study the immune environment associated with early tumor sites. While it was not surprising that the early immune response was predominated by macrophages and neutrophils, there were some novel findings at this early stage of disease. For instance, the macrophages and neutrophils expressed a mixed cytokine profile with TNF-α and TGF-β both produced at appreciable levels. Moreover, while the cells retained their phagocytic capacity, production of reactive oxygen species by the macrophages and neutrophils was in decline. Alterations in the metabolic profile of the tumor associated macrophages were also evident with a decrease in the ATP production rate, and a higher dependence on oxidative phosphorylation for ATP production. Collectively, these data indicate a mixed phenotype of tumor-associated macrophages and neutrophils evident within hours of murine mammary carcinoma delivery. |
| Databáze: | OpenAIRE |
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