Cytogenotoxicity evaluation of a heavy metal mixture, detected in a polluted urban wetland: Micronucleus and comet induction in the Indian green frog (Euphlyctis hexadactylus) erythrocytes and the Allium cepa bioassay

Autor: Uthpala A. Jayawardena, Preethi V. Udagama, Deepthi D. Wickramasinghe
Rok vydání: 2021
Předmět:
Environmental Engineering
Erythrocytes
Health
Toxicology and Mutagenesis
0208 environmental biotechnology
Comet
02 engineering and technology
010501 environmental sciences
medicine.disease_cause
Rana clamitans
01 natural sciences
Risk Assessment
Metal
Metals
Heavy
Onions
medicine
Environmental Chemistry
Bioassay
Animals
Ecosystem
0105 earth and related environmental sciences
Sri Lanka
biology
Chemistry
Public Health
Environmental and Occupational Health
food and beverages
General Medicine
General Chemistry
biology.organism_classification
Pollution
020801 environmental engineering
Environmental chemistry
visual_art
Wetlands
Micronucleus test
Euphlyctis hexadactylus
visual_art.visual_art_medium
Allium
Biological Assay
Comet Assay
Micronucleus
Genotoxicity
Water Pollutants
Chemical
Environmental Monitoring
Zdroj: Chemosphere. 277
ISSN: 1879-1298
Popis: Heavy metal contamination in wetland ecosystems is a serious environmental and health concern. This study evaluated the cytogenotoxicity of a previously evidenced heavy metal contamination (Cd, Cr, Cu, Pb and Zn ∼5 ppm each) in a polluted urban wetland, the Bellanwila-Attidiya sanctuary (BAS) in Sri Lanka, using a battery of cytogenotoxic assays. Micronucleus and comet assays evaluated the genotoxicity in erythrocytes of a common amphibian, the Indian green frog (Euphlyctis hexadactylus), under natural metal exposure in the wetland, and in vitro exposure, respectively.The Allium cepa bioassay assessed the cytogenotoxicity of the heavy metal mixture and of the individual metals, under laboratory exposure. Although in vivo natural exposure showed no significant induction of micronuclei in frog erythrocytes (P > 0.1), a significant and dose dependent elevation of comets was evident with in vitro exposure to the metal mixture (P Cd2+>Zn2+>Cr6 >Cu2+), mutagenic potential as evaluated in the percentage incidence of chromosomal aberrations (Pb2+> Zn2+> Cu2+> Cr6+> Cd2+) and cytotoxicity evaluated by the incidence of cell apoptosis and necrosis (Pb2+>Cr6+>Cu2+>Cd2+>Zn2+). Thus, the test battery of micronucleus, comet and A. cepa assays that reveal differential aspects of cytogenotoxicity may serve as a valuable tool in environmental monitoring, primarily to screen for complex environmental mixtures of heavy metals that may impact ecological health.
Databáze: OpenAIRE
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