Tumor necrosis factor receptor-associated protein 1 regulates cell adhesion and synaptic morphology via modulation of N-cadherin expression
| Autor: | Masaya Tohyama, M Tatsumi, Asako Kosuga, Norio Ozaki, Natsuko Kumamoto, Masatoshi Takeda, Kiyoshi Inoue, Ryota Hashimoto, Kyoko Kubota, Hiroshi Kunugi, Kunitoshi Kamijima, Nakao Iwata |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Dendritic spine Biology Biochemistry Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Antigens CD Internal medicine Cell Line Tumor medicine Cell Adhesion E2F1 Animals Humans HSP90 Heat-Shock Proteins Rats Wistar Cell adhesion STAT3 Cells Cultured Neurons Cadherin Tyrosine phosphorylation Cadherins Cell biology Rats Endocrinology chemistry Gene Expression Regulation Receptors Tumor Necrosis Factor Type I Synapses biology.protein Tumor necrosis factor alpha Signal transduction |
| Zdroj: | Journal of neurochemistry. 110(2) |
| ISSN: | 1471-4159 |
| Popis: | An increase in serum tumor necrosis factor-alpha (TNF-alpha) levels is closely related to the pathogenesis of major depression. However, the underlying molecular mechanism between this increase and impairment of brain function remains elusive. To better understand TNF-alpha/TNF receptor 1 signaling in the brain, we analyzed the brain distribution and function of tumor necrosis factor receptor-associated protein 1 (TRAP1). Here we show that TRAP1 is broadly expressed in neurons in the mouse brain, including regions that are implicated in the pathogenesis of major depression. We demonstrate that small interfering RNA-mediated knockdown of TRAP1 in a neuronal cell line decreases tyrosine phosphorylation of STAT3, followed by a reduction of the transcription factor E2F1, resulting in a down-regulation of N-cadherin, and affects the adhesive properties of the cells. In addition, in cultured hippocampal neurons, reduced expression of N-cadherin by TRAP1 knockdown influences the morphology of dendritic spines. We also report a significant association between several single nucleotide polymorphisms in the TRAP1 gene and major depression. Our findings indicate that TRAP1 mediates TNF-alpha/TNF receptor 1 signaling to modulate N-cadherin expression and to regulate cell adhesion and synaptic morphology, which may contribute to the pathogenesis of major depression. |
| Databáze: | OpenAIRE |
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