The C-terminal TPR domain of Tom70 defines a family of mitochondrial protein import receptors found only in animals and fungi
| Autor: | Trevor Lithgow, Nickie C Chan, Vladimir A Likic, Terrence D. Mulhern, Ross F. Waller |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Protein domain Receptors Cytoplasmic and Nuclear TIM/TOM complex In Vitro Techniques Mitochondrial Membrane Transport Proteins Conserved sequence Evolution Molecular Mitochondrial Proteins Mitochondrial membrane transport protein Structural Biology Mitochondrial Precursor Protein Import Complex Proteins Animals DNA Fungal Molecular Biology Conserved Sequence Phylogeny Genetics Binding Sites biology Base Sequence Fungal genetics Membrane Proteins DNA Translocon Mitochondria Protein Structure Tertiary Rats Tetratricopeptide Protein Transport Membrane protein biology.protein |
| Zdroj: | Monash University |
| ISSN: | 0022-2836 |
| Popis: | In fungi and animals the translocase in the outer mitochondrial membrane (TOM complex) consists of multiple components including the receptor subunit Tom70. Genome sequence analyses suggest no Tom70 receptor subunit exists in plants or protozoans, raising questions about its ancestry, function and the importance of its activity. Here we characterise the relationships within the Tom70 family of proteins. We find that in both fungi and animals, a conserved domain structure exists within the Tom70 family, with a transmembrane segment followed by 11 tetratricopeptide repeat motifs organised in three distinct domains. The C-terminal domain of Tom70 is highly conserved, and crucial for the import of hydrophobic substrate proteins, including those with and those without N-terminal presequences. Tom70 likely arose after fungi and animals diverged from other eukaryote lineages including plants, and subsequent gene duplication gave rise to a paralogue specific to the Saccharomyces group of yeasts. In animals and in fungi, Tom70 plays a fundamental role in the import of precursor proteins, by assisting relatively hydrophobic regions of substrate proteins into the translocation channel in the outer mitochondrial membrane. Proteins that function equivalently to Tom70 may have arisen independently in plants and protists. |
| Databáze: | OpenAIRE |
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