Quantification of Randomly-methylated-beta-cyclodextrin effect on liposome: an ESR study
| Autor: | Angeliki Grammenos, Maryse Hoebeke, Pierre-Henri Guelluy, Géraldine Piel, Mohamed Ali Bahri |
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| Rok vydání: | 2009 |
| Předmět: |
Lipid Bilayers
Biophysics Biochemistry Methylation Microviscosity Cyclic N-Oxides chemistry.chemical_compound Phosphatidylcholine Membrane fluidity Spin label Lipid bilayer Molecular Biology chemistry.chemical_classification Liposome Chromatography Cyclodextrin Chemistry Viscosity Bilayer beta-Cyclodextrins technology industry and agriculture Electron Spin Resonance Spectroscopy Cell Biology Cholesterol Liposomes lipids (amino acids peptides and proteins) Spin Labels Dimyristoylphosphatidylcholine |
| Zdroj: | Biochemical and biophysical research communications. 390(1) |
| ISSN: | 1090-2104 |
| Popis: | In the present work, the effect of Randomly-methylated-beta-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-l-alpha phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance (ESR) technique. The ability of Rameb to extract membrane cholesterol was demonstrated. For the first time, the percentage of cholesterol extracted by Rameb from cholesterol doped DMPC bilayer was monitored and quantified throughout a wide Rameb concentration range. The effect of cholesterol on the inner part of the membrane was also investigated using 16-doxyl stearic acid spin label (16-DSA). 16-DSA seems to explore two different membrane domains and report their respective microviscosities. ESR experiments also establish that the presence of 30% of cholesterol in DMPC liposomes suppresses the jump in membrane fluidity at lipids phase-transition temperature (23.9 degrees C). |
| Databáze: | OpenAIRE |
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