The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria
| Autor: | Jin Zehua, Zizhuo Tu, Caiyun Xie, Lei Fu, Meilin Jin, Li Han, Anding Zhang, Yaozu Xiang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Natronobacterium Sequence Homology Genome Integrity Repair and Replication Homology (biology) 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Genetics Recombinase Escherichia coli Homologous Recombination Gene 030304 developmental biology Gene Editing 0303 health sciences Aquifex aeolicus biology Thermus thermophilus Argonaute biology.organism_classification Cell biology Pyrococcus furiosus chemistry Prokaryotic Cells Argonaute Proteins 030217 neurology & neurosurgery DNA |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 |
| Popis: | Argonaute proteins are present and conserved in all domains of life. Recently characterized prokaryotic Argonaute proteins (pAgos) participates in host defense by DNA interference. Here, we report that the Natronobacterium gregoryi Argonaute (NgAgo) enhances gene insertions or deletions in Pasteurella multocida and Escherichia coli at efficiencies of 80–100%. Additionally, the effects are in a homologous arms-dependent but guide DNA- and potential enzyme activity-independent manner. Interestingly, such effects were also observed in other pAgos fragments including Thermus thermophilus Argonaute (TtAgo), Aquifex aeolicus Argonaute (AaAgo) and Pyrococcus furiosus Argonaute (PfAgo). The underlying mechanism of the NgAgo system is a positive selection process mainly through its PIWI-like domain interacting with recombinase A (recA) to enhance recA-mediated DNA strand exchange. Our study reveals a novel system for enhancing homologous sequence-guided gene editing in bacteria. |
| Databáze: | OpenAIRE |
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