The purification of SP1-β (pregnancy-specific β1-glycoprotein) using immobilized triazine dyes
| Autor: | Kevin G. McFarthing, Sarojani Angal, Peter D.G. Dean |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
chemistry.chemical_classification Chromatography Cibacron blue Triazines Chemistry Immune Sera Pregnancy-Specific beta 1-Glycoproteins Biophysics Cell Biology Biochemistry chemistry.chemical_compound Procion red Pregnancy Animals Humans Electrophoresis Polyacrylamide Gel Female Rabbits Coloring Agents Glycoprotein Molecular Biology Triazine |
| Zdroj: | Analytical Biochemistry. 122:186-193 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(82)90269-x |
| Popis: | Pregnancy-specific β 1 2 -glycoprotein was purified to 99% purity by sequential chromatography on carboxymethyl-cellulose (to remove other pregnancy-specific β 1 -glycoprotein variants), Cibacron blue 3G-A-Matrex Gel, Procion turquoise MX-G-Matrex Gel, Procion red H-8BN-Matrex Gel, and rabbit anti-normal human male serum-Matrex Gel. The purification was rapid, large-scale, and gave a yield of pregnancy-specific β 1 -glycoprotein of 35.1%. |
| Databáze: | OpenAIRE |
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