Effects of mechanical loading on collagen propeptides processing in cartilage repair
| Autor: | Stefan Marlovits, Rosmarie Hardmeier, Heinz Redl |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cartilage
Articular Biomedical Engineering Medicine (miscellaneous) Biocompatible Materials Matrix (biology) Models Biological Chondrocyte Biomaterials Extracellular matrix Chondrocytes Tissue engineering Cartilage transplantation medicine Animals Humans Tissue Engineering Tissue Scaffolds Chemistry Cartilage Procollagen N-Endopeptidase Cell biology Biomechanical Phenomena Procollagen peptidase medicine.anatomical_structure Collagen Protein Processing Post-Translational Procollagen Biomedical engineering |
| Zdroj: | Journal of tissue engineering and regenerative medicine. 4(1) |
| ISSN: | 1932-7005 |
| Popis: | Injured articular cartilage has poor reparative capabilities and if left untreated may develop into osteoarthritis. Unsatisfactory results with conventional treatment methods have brought as an alternative treatment the development of matrix autologous chondrocyte transplants (MACTs). Recent evidence proposes that the maintenance of the original phenotype by isolated chondrocytes grown in a scaffold transplant is linked to mechanical compression, because macromolecules, particularly collagen, of the extracellular matrix have the ability to 'self-assemble'. In load-bearing tissues, collagen is abundantly present and mechanical properties depend on the collagen fibre architecture. Study of the active changes in collagen architecture is the focus of diverse fields of research, including developmental biology, biomechanics and tissue engineering. In this review, the structural model of collagen assembly is presented in order to understand how scaffold geometry plays a critical role in collagen propeptide processing and chondrocyte development. When physical forces are applied to different cell-based scaffolds, the resulting specific twist of the scaffolds might be accompanied by changes in the fibril pattern synthesis of the new collagen. The alteration in the scaffolds due to mechanical stress is associated with cellular signalling communication and the preservation of N-terminus procollagen moieties, which would regulate both the collagen synthesis and the diameter of the fibre. The structural difference would also affect actin stabilization, cytoskeleton remodelling and proteoglycan assembly. These effects seemed to be dependent on the magnitude and duration of the physical stress. This review will contribute to the understanding of mechanisms for collagen assembly in both a natural and an artificial environment. |
| Databáze: | OpenAIRE |
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