Roles of the Peptide Transport Systems and Aminopeptidase PepA in Peptide Assimilation by Helicobacter pylori
| Autor: | Soon-Kyu Yun, Ji Hyun Lee, Se-Young Hwang, Kyung-Min Choi, Mi Ran Ki |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Peptide
medicine.disease_cause Real-Time Polymerase Chain Reaction Applied Microbiology and Biotechnology Aminopeptidase Aminopeptidases Microbiology chemistry.chemical_compound Western blot medicine Escherichia coli chemistry.chemical_classification PEPA medicine.diagnostic_test biology Helicobacter pylori Gene Expression Profiling Hydrolysis Membrane Transport Proteins General Medicine Dipeptides biology.organism_classification Anti-Bacterial Agents chemistry Biochemistry Peptide transport Growth inhibition Bacteria Biotechnology |
| Zdroj: | Journal of microbiology and biotechnology. 25(10) |
| ISSN: | 1738-8872 |
| Popis: | Peptide assimilation in Helicobacter pylori necessitates a coordinated working of the peptide transport systems (PepTs) and aminopeptidase (PepA). We found that H. pylori hydrolyzes two detector peptides, L-phenylalanyl-L-3-thiaphenylalanine (PSP) and L-phenylalanyl-L-2- sulfanilylglycine (PSG), primarily before intake and excludes their antibacterial effects, whereas Escherichia coli readily transports them with resultant growth inhibition. PSP assimilation by H. pylori was inhibited by aminopeptidase inhibitor bestatin, but not by dialanine or cyanide-m-chlorophenylhydrazone, contrary to that of E. coli. RT- and qRT-PCR analyses showed that H. pylori may express first the PepTs (e.g., DppA and DppB) and then PepA. In addition, western blot analysis of PepA suggested that the bacterium secretes PepA in response to specific inducers. |
| Databáze: | OpenAIRE |
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