Dissemination of VanA-TypeEnterococcus faeciumIsolates in Hungary
| Autor: | Szilvia Melegh, Tamás Kovács, Virág Lesinszki, Zsuzsanna Dombrádi, Ágoston Ghidán, Judit Szabó, Judit Pászti, Gyula Mestyán, Brigitta Berta, Krisztina Kovacs, Ákos Tóth, Adrienn Nyul |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Microbiology (medical) Pharmacology Vancomycin resistance Hungary biology Molecular epidemiology Enterococcus faecium 030106 microbiology Immunology Vancomycin Resistance biochemical phenomena metabolism and nutrition biology.organism_classification Microbiology Anti-Bacterial Agents 03 medical and health sciences Bacterial Proteins Humans bacteria Gram-Positive Bacterial Infections Multilocus Sequence Typing Plasmids |
| Zdroj: | Microbial Drug Resistance. 24:1376-1390 |
| ISSN: | 1931-8448 1076-6294 |
| Popis: | Although vanA carrying Enterococcus faecium human clinical isolates have been rarely found in Hungary before 2012, they have been detected in continuously increasing numbers since then. To identify factors associated with their dissemination, we investigated the clonal relatedness and plasmids of 30 vanA carrying E. faecium isolates originating from different Hungarian healthcare institutions from 2012 to 2014. Molecular typing of the isolates (n = 30) was performed with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, Tn1546 polymerase chain reaction mapping, plasmid restriction fragment length polymorphism analysis, and sequencing. A single Tn1546 variant was detected in all of the isolates. It harbored IS1251 in the vanS-vanH intergenic region, had an entire deletion of the transposase gene and a partial deletion of the resolvase gene, and was located on a pRUM-like plasmid. Based on PFGE, the isolates could be grouped into 13 pulsotypes. Representative strains of these pulsotypes belonged to ST17, ST18, ST80, ST117, and ST203, which are known to be part of the hospital-adapted clades. The increase in the number of vanA carrying E. faecium clinical isolates in Hungary could be explained by the dissemination of pRUM-like vancomycin resistance plasmids in hospital-adapted clonal lineages. |
| Databáze: | OpenAIRE |
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