Three-dimensional and non-destructive characterization of nerves inside conduits using laboratory-based micro computed tomography
| Autor: | Daniel F. Kalbermatten, Lucas Degrugillier, Bert Müller, Simone E. Hieber, Srinivas Madduri, Peter Thalmann, Georg Schulz, Christos Bikis |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Nerve segmentation Computed tomography computer.software_genre X-ray Rats Sprague-Dawley 03 medical and health sciences Imaging Three-Dimensional 3D imaging Peripheral nerve Voxel ΜCT Non destructive medicine Animals Histocytological Preparation Techniques ddc:617 medicine.diagnostic_test General Neuroscience Micro computed tomography fungi Vessel assessment Anatomy X-Ray Microtomography Sciatic Nerve 030104 developmental biology Artifacts computer Biomedical engineering |
| Zdroj: | Journal of Neuroscience Methods, Vol. 294 (2018) pp. 59-66 |
| ISSN: | 1872-678X 0165-0270 |
| Popis: | Background Histological assessment of peripheral nerve regeneration in animals is tedious, time-consuming and challenging for three-dimensional analysis. New method The present study reports on how and to what extent micro computed tomography of paraffin-embedded samples can provide a reliable three-dimensional approach for quantitative analysis of peripheral nerves. Results Rat sciatic nerves were harvested, formalin-fixated, positioned into nerve conduits (NC), paraffin-embedded, and imaged using a laboratory-based X-ray microtomography system with an isotropic voxel length of 4 μm. Suitable quantitative measures were identified and automatically evaluated, i.e. nerve length, cross-sectional area and volume, as well as vascular structures, to be used as an assessment and comparison indicator of regeneration quality. Comparison with existing methods Compared to imaging using contrast agents, the investigated specimens can subsequently undergo the conventional histological analysis without requiring additional preparation steps. Contrast and spatial resolution are also increased significantly. Conclusions We demonstrate the potential of the micro computed tomography for non-destructive monitoring of peripheral nerves inside the conduits. |
| Databáze: | OpenAIRE |
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