Calmodulin-independent nitric oxide synthase from rat polymorphonuclear neutrophils
| Autor: | S Terao, Kunihiko Kosuga, S Ohkawa, Kazuaki Hiki, Hiroshi Eizawa, Yoshiki Yui, Chuichi Kawai, Ryuichi Hattori, K Ohnishi |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Calmodulin Neutrophils Biochemistry Chromatography Affinity Cofactor Affinity chromatography Animals Molecular Biology Polyacrylamide gel electrophoresis Gel electrophoresis chemistry.chemical_classification biology Rats Inbred Strains Cell Biology Enzyme assay Rats Nitric oxide synthase Enzyme chemistry biology.protein Electrophoresis Polyacrylamide Gel Amino Acid Oxidoreductases Nitric Oxide Synthase |
| Zdroj: | Journal of Biological Chemistry. 266:3369-3371 |
| ISSN: | 0021-9258 |
| Popis: | Recently, the purification of nitric oxide synthase (EC 1.14.23) from rat cerebellum has been reported, and the enzyme is a calmodulin-requiring enzyme (Bredt, D. S., and Snyder, S. H. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 682-685). In this paper, nitric oxide synthase has been purified to near homogeneity from the cytosol fraction of rat polymorphonuclear neutrophils. The purification procedure involves affinity chromatography with adenosine 2',5'-diphosphate-agarose and an anion exchange column, DEAE-Bio-Gel A. On polyacrylamide gel electrophoresis in sodium dodecyl sulfate, the enzyme migrated as a single protein band with Mr = 150,000. The molecular weight was estimated to be 150,000 by gel filtration on a Superose 12 HR 10/30. The purified enzyme was unstable with a half-life of 3 h at pH 7.4 and 4 degrees C. The enzyme activity required the presence of Ca2+, NADPH, FAD, and (6R)-5,6,7,8-tetrahydro-L-biopterin. Calmodulin antagonists (W5, W7, W13, and trifluoperazine dihydrochloride) did not inhibit the enzyme activity, and the addition of calmodulin was also ineffective for the increase in the enzyme activity. The neutrophil enzyme appears to be a calmodulin-independent type of nitric oxide synthase. |
| Databáze: | OpenAIRE |
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