Anti-edema effect of melatonin on spinal cord injury in rats
| Autor: | Jishun Yang, Liang-Bi Xiang, Ming-xiao Hou, Xinwei Liu, Yun-En Liu, Yu Wang, Dapeng Zhou |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_treatment lcsh:Medicine Down-Regulation Antioxidants General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley Sham group Melatonin Random Allocation Body Water Edema Glial Fibrillary Acidic Protein medicine Animals Spinal cord injury Saline Spinal Cord Injuries Aquaporin 4 business.industry lcsh:R Laminectomy medicine.disease Spinal cord spinal cord injury rats medicine.anatomical_structure Spinal Cord Anesthesia Immunohistochemistry medicine.symptom business medicine.drug |
| Zdroj: | Biomedical Papers, Vol 159, Iss 2, Pp 220-226 (2015) |
| ISSN: | 1804-7521 1213-8118 |
| DOI: | 10.5507/bp.2015.012 |
| Popis: | Aim: To determine the anti-edema effects of melatonin on spinal cord injury (SCI) in rats. Methods: A total of 150 adult male Sprague-Dawley rats were randomly allocated to the following three groups (n=50): a sham group which underwent laminectomy without dural compression; an SCI group, which underwent laminectomy followed by SCI and received saline i.p. immediately after injury and then daily for 2 days; an MT group, which underwent laminectomy followed by SCI and received a 100 mg/kg dose of melatonin i.p. immediately after SCI and then daily for 2 days. The cords were removed at 12, 24, 48 and 72 h after surgery in every group. Spinal cord edema was evaluated by determining the spinal cord water content. Expressions of AQP4 and GFAP positive cells in injured spinal cord were detected by immunohistochemical staining, and protein expressions of AQP4 and GFAP were detected by Western blotting. Results: Spinal cord water content was obviously increased after SCI, which was maintained almost unchanged by melatonin treatment (100 mg/kg) at 12 h after injury but was significantly reduced from 24 h to 72 h. The expressions of AQP4 and GFAP increased in the injured spinal cord segments, which were decreased by melatonin treatment (100 mg/kg) between 24 h and 72 h after SCI. Conclusions: Melatonin (100 mg/kg) had anti-edema effects after acute SCI probably by down-regulating the expression level of AQP4 protein, and it may eliminate astrocytic swelling after SCI through down-regulating the expression level of GFAP protein. |
| Databáze: | OpenAIRE |
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