Does the applicability ofBacillusstrains in probiotics rely upon their taxonomy?
| Autor: | Safronova La, V V Klochko, L. B. Zelena, Oleg N. Reva |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Bacillus amyloliquefaciens
Probiotics Immunology Proteolytic enzymes Bacillus General Medicine Biology 16S ribosomal RNA biology.organism_classification Applied Microbiology and Biotechnology Microbiology Lacticaseibacillus casei Broad spectrum RNA Ribosomal 16S Genetics Animals Taxonomy (biology) Molecular Biology Phylogeny Bacillus velezensis |
| Zdroj: | Canadian Journal of Microbiology. 58:212-219 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/w11-113 |
| Popis: | The taxonomic position and biological activities of two Bacillus strains used in veterinary probiotics were studied in this work. These microorganisms inhibit growth of a broad spectrum of pathogenic cultures. They synthesize proteolytic enzymes and other biologically active metabolites, and to some extent, supplement each other with probiotic activities. It is not clear whether these versatile activities are properties of individual strains or bacterial taxa as whole. 16S rRNA compari- sons were conducted and illustrated the relatedness of these strains to Bacillus amyloliquefaciens. Their cell wall fatty acid contents were consequently analysed and specified a relation to the "Bacillus velezensis" ecomorph. On account of the pre- vious observations, a simple method of 16S rRNA profiling by polymorphic nucleotides was proposed to determine a group of organisms closely related to "B. velezensis" and B. amyloliquefaciens subsp. plantarum, for they are biologically active strains suitable for use in biotechnology. The extreme genetic plasticity of these bacteria endowed each strain with a unique spectrum of antagonistic activity. Resume : La position taxonomique et les activites biologiques de deux souches de Bacillus utilisees comme probiotiques a usage veterinaire ont ete etudiees dans cette etude. Ces microorganismes inhibent la croissance d'un large spectre de cultures de pathogenes. Ils synthetisent des enzymes proteolytiques et d'autres metabolites actifs biologiquement et, a un certain de- gre, se complementent quant a leurs activites probiotiques. On ne sait pas precisement si ces activites versatiles sont des proprietes des souches individuelles ou du taxon bacterien dans son ensemble. Les comparaisons de sequences d'ARNr 16S ont ete realisees et illustraient le degre de relation entre ces souches et Bacillus amyloliquefaciens. Le contenu de leur paroi cellulaire en acides gras a ete consequemment analyse et a montre une relation avec l'ecomorphe "Bacillus velezensis" .E n raison des observations precedentes, une simple methode de profil de l'ARNr 16S a l'aide de nucleotides polymorphiques a ete proposee pour identifier un groupe d'organismes etroitement relies a "B. velezensis" et B. amyloliquifaciens subsp. plan- tarum, car elles constituent des souches biologiquement actives, adequates pour une utilisation en biotechnologie. La plasti- cite genetique extreme de ces bacteries dote chaque souche d'un spectre unique d'activites antagonistes. Mots-cles : Bacillus, probiotique, phylogenetique microbienne, activite antimicrobienne. (Traduit par la Redaction) |
| Databáze: | OpenAIRE |
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