Cyclin D1 overexpression increases susceptibility to 4-nitroquinoline-1-oxide-induced dysplasia and neoplasia in murine squamous oral epithelium
| Autor: | Sanjay M. Mallya, Salony M. Patel, Glenn K Buchberger, Anil K. Rustgi, Hiroshi Nakagawa, Ellen Eisenberg, Carmen Z. Michaylira, Jonathan F. Wilkey, Kirsten Saucier |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Cancer Research Epithelial dysplasia Cyclin B Gene Expression Mice Transgenic Biology medicine.disease_cause Epithelium Article Mice Cyclin D1 medicine Animals Genetic Predisposition to Disease Transgenes Molecular Biology Cyclin-Dependent Kinase Inhibitor p16 Cell Proliferation Mouth neoplasm Oncogene Mouth Mucosa Cancer Cell Differentiation Blotting Northern medicine.disease Immunohistochemistry 4-Nitroquinoline-1-oxide Disease Models Animal Ki-67 Antigen Dysplasia Carcinogens biology.protein Cancer research Keratin-5 Female Mouth Neoplasms Carcinogenesis Precancerous Conditions |
| Zdroj: | Molecular Carcinogenesis. 48:853-861 |
| ISSN: | 1098-2744 0899-1987 |
| DOI: | 10.1002/mc.20531 |
| Popis: | The cyclin D1 oncogene is frequently amplified/overexpressed in oral squamous cell carcinomas. Mice with overexpression of cyclin D1 targeted to the stratified squamous epithelia of the tongue, esophagus, and forestomach develop a phenotype of epithelial dysplasia at these sites. In this study, we examined the effect of cyclin D1 overexpression on susceptibility of mice to carcinogen-induced tumorigenesis, using 4-nitroquinoline-1-oxide (4NQO), an established potent oral carcinogen in mice. Cyclin D1 overexpressing mice and nontransgenic littermates were administered 4NQO (20 or 50 parts per million (ppm) in the drinking water) for 8 wk and monitored for an additional 16 wk. Histopathological analyses of the tongue revealed significantly higher severity of dysplasia in the cyclin D1 overexpression mice, compared with nontransgenic controls and with untreated controls. Moreover, only the cyclin D1 overexpression mice developed neoplastic lesions in the oro-esophageal epithelia. Examination of the dysplastic and neoplastic lesions revealed abnormal proliferation. Our findings suggest that cyclin D1 overexpression enhances susceptibility to carcinogen-induced oral tumorigenesis. These results underscore the importance of cyclin D1 in the process of oral neoplastic development. Further, they emphasize the value of this transgenic model to study the pathogenesis of oral precancer and cancer and establish it as a model system to test candidate agents for chemoprevention of upper aero-digestive cancer. |
| Databáze: | OpenAIRE |
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