Regulation of 5-oxo-ETE synthesis by nitric oxide in human polymorphonuclear leucocytes upon their interaction with zymosan and Salmonella typhimurium

Autor: Julia M. Romanova, Galina M. Viryasova, Galina F. Sud'ina, Svetlana I. Galkina, Tatjana V. Gaponova
Rok vydání: 2014
Předmět:
Male
Salmonella typhimurium
HBSS
Hanks balanced salt solution
Salmonella
Lipopolysaccharide
Neutrophils
5-LOX
5-lipoxygenase
5-HEDH
5-hydroxyeicosanoid dehydrogenase
lcsh:Life
lcsh:QR1-502
AA
arachidonic acid
medicine.disease_cause
G6PD
glucose-6-phosphate dehydrogenase
Biochemistry
lcsh:Microbiology
chemistry.chemical_compound
mGC
membrane-bound form of GC
sGC
soluble guanylyl cyclase
ANF
atrial natriuretic peptide
Leukotriene
leukotriene
Dnp-Cl
1-chloro-2
4-dinitrobenzene
OZ
opsonized zymosan
neutrophil
5-oxo-ETE
Tetradecanoylphorbol Acetate
LPS
lipopolysaccharide
Female
Arachidonic acid
PMA
phorbol 12-myristate 13-acetate
LTB4
leukotriene B4
DEA NONOate
1
1-diethyl-2-hydroxy-2-nitroso-hydrazine sodium
LT
leukotriene
HBSS/Hepes
Hanks balanced salts medium containing 10 mM Hepes
Biophysics
Arachidonic Acids
Nitric Oxide
YC-1
3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole
S4
Nitric oxide
5-oxo-ETE
5-oxo-6
8
11
14-eicosatetraenoic acid
ROS
reactive oxygen species
medicine
Humans
PMNL
polymorphonuclear leucocyte
Molecular Biology
Opsonin
Original Paper
NO
nitric oxide
Zymosan
PGB2
prostaglandin B2
Cell Biology
lipoxygenase
G-CSF
granulocyte colony-stimulating factor
Molecular biology
lcsh:QH501-531
GM-CSF
granulocyte/macrophage colony-stimulating factor
chemistry
Carcinogens
5-HETE
5S-hydroxy-6
8
11
14-eicosatetraenoic acid
Phorbol
Zdroj: Bioscience Reports
Bioscience Reports, Vol 34, Iss 3, p e00108 (2014)
ISSN: 1573-4935
0144-8463
Popis: In the present study we have presented data on the regulation of LT (leukotriene) and 5-oxo-ETE (5-oxo-6,8,11,14-eicosatetraenoic acid) syntheses in human neutrophils upon interaction with OZ (opsonized zymosan) or Salmonella typhimurium. Priming of neutrophils with PMA (phorbol 12-myristate 13-acetate) and LPS (lipopolysaccharide) elicits 5-oxo-ETE formation in neutrophils exposed to OZ, and the addition of AA (arachidonic acid) significantly increases 5-oxo-ETE synthesis. We found that NO (nitric oxide)-releasing compounds induce 5-oxo-ETE synthesis in neutrophils treated with OZ or S. typhimurium. Exposure of neutrophils to zymosan or bacteria in the presence of the NO donor DEA NONOate (1,1-diethyl-2-hydroxy-2-nitroso-hydrazine sodium) considerably increased the conversion of endogenously formed 5-HETE (5S-hydroxy-6,8,11,14-eicosatetraenoic acid) to 5-oxo-ETE. To our knowledge, this study is the first to demonstrate that NO is a potent regulator of 5-oxo-ETE synthesis in human polymorphonuclear leucocytes exposed to Salmonella typhimurium and zymosan.
Nitric oxide significantly increased 5-oxo-ETE formation in neutrophils. 5-oxo-ETE is a key 5-lipoxygenase metabolite in human polymorphonuclear leucocytes exposed to NO upon interaction with opsonized zymosan or Salmonella typhimurium.
Databáze: OpenAIRE
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