Characterization and high-resolution mapping of a late blight resistance locus similar to R2 in potato
| Autor: | H.J. van Eck, E.A.G. van der Vossen, Tae-Ho Park, Vivianne G. A. A. Vleeshouwers, Richard G. F. Visser, D. J. Huigen |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Phytophthora
DNA Plant Population Locus (genetics) solanum-tuberosum broad-spectrum resistance Genes Plant Solanum aflp markers Laboratorium voor Plantenveredeling Gene mapping Species Specificity PRI Biodiversiteit en Veredeling Gene cluster Genetics education Plant Diseases Solanum tuberosum globodera-pallida education.field_of_study phytophthora-infestans mont biology Gene map EPS-2 chromosome-v virus-x Bulked segregant analysis Chromosome Mapping food and beverages General Medicine R gene biology.organism_classification gene-cluster PRI Biodiversity and Breeding Plant Breeding Phytophthora infestans Agronomy and Crop Science hypersensitive resistance race-specific resistance Biotechnology |
| Zdroj: | Theoretical and Applied Genetics, 111(3), 591-597 Theoretical and Applied Genetics 111 (2005) 3 |
| ISSN: | 0040-5752 |
| Popis: | Identification of resistance (R) genes to Phytophthora infestans is an essential step in molecular breeding of potato. We identified three specific R genes segregating in a diploid mapping population. One of the R genes is located on chromosome 4 and proved phenotypically indistinguishable from the Solanum demissum-derived R2, although S. demissum is not directly involved in the pedigree of the population. By bulked segregant analysis combined with a resistance assay, a genetic linkage map of the R2-like locus was constructed with 30 coupling and 23 repulsion phase AFLP markers. Two markers flanking the R2-like locus were applied to screen an extended population of 1,586 offspring. About 103 recombinants were selected, and an accurate high-resolution map was constructed. The R2-like resistance was localized in a 0.4 cM interval and was found co-segregating with four AFLP markers, which can be used to isolate the R2-like gene by map-based gene cloning. By analyzing race-specificity and R gene-specific molecular markers, we also found that an R1-like gene and an additional unknown R gene are segregating in the population. |
| Databáze: | OpenAIRE |
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