Molecular forms of myeloperoxidase in human plasma
| Autor: | Clive Little, T Holm, R L Olsen, T K Steigen |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Gel electrophoresis
Antigenicity biology Chemistry Immunochemistry Protein subunit Radioimmunoassay Cell Biology Chromatography Ion Exchange Biochemistry Chromatography Affinity Peptide Fragments Affinity chromatography Spectrophotometry Myeloperoxidase biology.protein Humans Electrophoresis Polyacrylamide Gel Molecular Biology Eosinophil peroxidase Peroxidase Research Article |
| Zdroj: | Biochemical Journal. 237:559-565 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2370559 |
| Popis: | A radioimmunoassay for myeloperoxidase was established with the use of affinity-purified anti-(human myeloperoxidase) immunoglobulins. By the use of ion-exchange followed by immunoaffinity chromatography a preparation of immunoreactive, catalytically active myeloperoxidase was obtained from fresh human plasma. In non-denaturing gel electrophoresis, the plasma preparation showed about four catalytically active components of mobility very similar to that of the granulocyte enzyme. SDS/polyacrylamide-gel electrophoresis combined with protein blotting showed that the two polypeptides of strongest antigenicity in the plasma preparation corresponded in Mr to the large and the small subunits of the granulocyte enzyme. In addition, the plasma preparation contained a higher-Mr immunoreactive polypeptide, possibly a precursor form of the enzyme, together with another of Mr similar to that of the large subunit of eosinophil peroxidase. |
| Databáze: | OpenAIRE |
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