Effect of the Contents and Form of Rabies Glycoprotein on the Potency of Rabies Vaccination in Cattle
| Autor: | G. M. M. Caporale, M. T. Terreran, K. M. S. Pieri, Carlos R. Zanetti, G. M. Lusa, A. T. Piza, L. A. Machado |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine Immunogen lcsh:RC955-962 lcsh:QR1-502 Cattle Diseases Enzyme-Linked Immunosorbent Assay rabies Biology Antibodies Viral lcsh:Microbiology Mice Rabies vaccine Viral Envelope Proteins Neutralization Tests medicine Animals Potency Antigens Viral Glycoproteins Immunogenicity NIH test medicine.disease Virology Vaccination Titer Rabies Vaccines biology.protein rabies glycoprotein Cattle Rabies Antibody cattle vaccination medicine.drug |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 97, Iss 2, Pp 265-268 (2002) Memórias do Instituto Oswaldo Cruz, Volume: 97, Issue: 2, Pages: 265-268, Published: MAR 2002 |
| ISSN: | 0074-0276 |
| Popis: | One of the methods used for controlling cattle rabies in Brazil consists of vaccination. Sometimes, however, rabies occurs in cattle supposedly protected. Since rabies vaccine batches are officially controlled by tests performed on laboratory animals, it is questionable whether the minimal mandatory requirements really correspond to immunogenicity in the target species. We have analyzed the association among potencies of rabies vaccines tested by the NIH test, the contents and form (free-soluble or virus-attached) of rabies glycoprotein (G) in the vaccine batches, and the virus-neutralizing antibodies (VNA) titers elicited in cattle. No correlation was found between G contents in the vaccine batches and the NIH values, whatever the presentation of G. There was no correlation either between NIH values and VNA titers elicited in cattle. There was, however, a positive correlation (r = 0.8681; p = 0.0001) between the amounts of virion-attached G present in the vaccine batches and VNA elicited in cattle. This was not observed when the same analysis was performed with total-glycoprotein or free-soluble glycoprotein. The study demonstrated that NIH values can not predict the effect of the immunogen in cattle. On the other hand, the quantification of virus-attached rabies glycoprotein has a strong correlation with VNA elicited in cattle. |
| Databáze: | OpenAIRE |
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