Cancer-Associated Fibroblasts Autophagy Enhances Progression of Triple-Negative Breast Cancer Cells
| Autor: | Mengchuan Wang, Kankan Zhao, Guoli Shao, Yi-Zhe Huang, Shufeng Ji, Zixiang Wang, Jian Zhang, Aiguo Wu, Shaobo Feng, Danxun Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
China Epithelial-Mesenchymal Transition Myocytes Smooth Muscle Vimentin Triple Negative Breast Neoplasms Biology Metastasis 03 medical and health sciences 0302 clinical medicine Western blot Cancer-Associated Fibroblasts Lab/In Vitro Research Cell Movement Cell Line Tumor medicine Autophagy Humans MTT assay Epithelial–mesenchymal transition Wnt Signaling Pathway Triple-negative breast cancer beta Catenin Cell Proliferation medicine.diagnostic_test Cell Cycle Wnt signaling pathway General Medicine Fibroblasts medicine.disease Cadherins Molecular biology Actins 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Disease Progression Female |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 1234-1010 |
| Popis: | BACKGROUND Cancer-associated fibroblasts (CAFs) are key factors in malignant tumor initiation, progression, and metastasis. However, the effect of CAFs autophagy on triple-negative breast cancer (TNBC) cells is not clear. In this study, the growth effect of TNBC cells regulated by CAFs autophagy was evaluated. MATERIAL AND METHODS CAFs were obtained from invasive TNBC tumors and identified by Western blot and immunofluorescence staining assay. CAFs were co-cultured with TNBC cells, and migration and invasion were evaluated by Matrigel-coated Transwell and Transwell inserts. TNBC cells growth was detected by MTT assay, and epithelial-mesenchymal transition (EMT) regulated by CAFs was evaluated by Western blot assay. RESULTS CAFs were identified by the high expression of α-smooth muscle actin (α-SMA) protein. Autophagy-relevant Beclin 1 and LC3-II/I protein conversion levels in CAFs were higher than those in NFs (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|