Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells
| Autor: | Yong Hee Rhee, Rae Kwon Kim, Su Jae Lee, Sang Hoon Yi, Hyongbum Kim, Mi Yoon Chang, Chang-Hwan Park, Sang Hun Lee |
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| Rok vydání: | 2014 |
| Předmět: |
Pluripotent Stem Cells
Cell Survival Cellular differentiation Biology Biochemistry Article Phosphatidylinositol 3-Kinases Neural Stem Cells Genetics medicine Humans Induced pluripotent stem cell lcsh:QH301-705.5 Cells Cultured Embryonic Stem Cells Cell Proliferation Antibacterial agent Neurons Doxycycline lcsh:R5-920 Cell growth Cell Differentiation Cell Biology Embryonic stem cell Neural stem cell Anti-Bacterial Agents Cell biology lcsh:Biology (General) Karyotyping Stem cell lcsh:Medicine (General) Proto-Oncogene Proteins c-akt Signal Transduction Developmental Biology medicine.drug |
| Zdroj: | Stem Cell Reports Stem Cell Reports, Vol 3, Iss 2, Pp 353-364 (2014) STEM CELL REPORTS(3): 2 |
| ISSN: | 2213-6711 |
| DOI: | 10.1016/j.stemcr.2014.06.013 |
| Popis: | Summary We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance. Highlights • Doxycycline enhances human pluripotent ESC/iPSC survival and self-renewal • Doxycycline-mediated survival effects are also shown in human neural stem cell (NSC) • Doxycycline effects are not associated with its antibacterial action • The doxycycline actions are mediated by direct activation of a PI3K-Akt signaling In this article, Lee and colleagues show that doxycycline, an antibacterial agent, can be used as a useful culture supplement for facilitating cell survival and self-renewal of human embryonic stem and induced pluripotent stem cells (hESC/iPSCs). These doxycycline effects are not associated with its antibacterial action but mediated by direct activation of a PI3K-Akt intracellular signal. |
| Databáze: | OpenAIRE |
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