Modulation of sympathetic preganglionic neuron activity via adrenergic receptors

Autor: Kamon Iigaya, Youichi Kobayashi, Hiroshi Onimaru, Yoshino Minoura
Rok vydání: 2018
Předmět:
0301 basic medicine
medicine.medical_specialty
Sympathetic nervous system
Patch-Clamp Techniques
Sympathetic Nervous System
Physiology
Action Potentials
Inhibitory postsynaptic potential
Norepinephrine
03 medical and health sciences
0302 clinical medicine
Postsynaptic potential
Internal medicine
Adrenergic alpha-2 Receptor Agonists
Internal Medicine
medicine
Animals
Patch clamp
Neurons
Membrane potential
Chemistry
musculoskeletal
neural
and ocular physiology
Excitatory Postsynaptic Potentials
Depolarization
Hyperpolarization (biology)
Rats
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Inhibitory Postsynaptic Potentials
Spinal Cord
nervous system
Excitatory postsynaptic potential
Cardiology and Cardiovascular Medicine
Adrenergic alpha-Agonists
Dexmedetomidine
030217 neurology & neurosurgery
Zdroj: Hypertension Research. 41:499-505
ISSN: 1348-4214
0916-9636
DOI: 10.1038/s41440-018-0049-x
Popis: The sympathetic preganglionic neurons (SPNs) play a key role in the sympathetic nervous system. Previous reports have suggested that norepinephrine (NE) directly affects SPNs via both inhibitory hyperpolarization interactions mediated by α2 receptors and excitatory depolarization interactions mediated by α1 receptors. It remains poorly understood, however, whether the excitability of SPNs can be inhibited indirectly (presynaptically) as well as directly (postsynaptically). We intracellularly recorded 41 SPNs using the whole-cell patch-clamp technique in spinal cord slice preparations of neonatal rats. We examined the effects of NE or dexmedetomidine hydrochloride (Dxm) (α2-adrenergic receptor agonist) on SPNs by analyzing the excitatory postsynaptic potentials (EPSPs) and inhibitory postsynaptic potentials (IPSPs). EPSPs were dominant in 15 SPNs (EPSP-SPNs) and IPSPs were dominant in 7 SPNs (IPSP-SPNs) at baseline. We were unable to analyze the postsynaptic potentials in the other 19 SPNs, due to high frequency of action potential firings (firing-SPNs). At baseline, the membrane potentials and resistances of each type of SPN were similar. NE (1 μM) gradually depolarized the EPSP-SPNs and IPSP-SPNs (P
Databáze: OpenAIRE
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