Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson’s Disease

Autor: Maria Eugenia Bernis, Gültekin Tamgüney
Přispěvatelé: Ripp, Steven
Rok vydání: 2019
Předmět:
0301 basic medicine
Parkinson's disease
animal diseases
metabolism [Parkinson Disease]
Fluorescent Antibody Technique
Mice
Transgenic
methods [Luminescent Measurements]
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
ddc:570
methods [Molecular Imaging]
diagnostic imaging [Parkinson Disease]
Animals
Humans
Medicine
Bioluminescence imaging
Luciferase
metabolism [alpha-Synuclein]
Pathological
Neuroinflammation
pathology [Inflammation]
Mice
Knockout
Alpha-synuclein
Synucleinopathies
metabolism [Inflammation]
business.industry
medicine.disease
pathology [Parkinson Disease]
nervous system diseases
Astrogliosis
Disease Models
Animal
030104 developmental biology
nervous system
chemistry
metabolism [Neurons]
etiology [Parkinson Disease]
business
Neuroscience
Biomarkers
030217 neurology & neurosurgery
Zdroj: Methods in Molecular Biology ISBN: 9781493999392
New York, NY : Springer US, Methods in Molecular Biology 2081, 147-159 (2020). doi:10.1007/978-1-4939-9940-8_10
Bioluminescent Imaging / Ripp, Steven (Editor) ; New York, NY : Springer US, 2020, Chapter 10 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-9939-2=978-1-4939-9940-8 ; doi:10.1007/978-1-4939-9940-8
DOI: 10.1007/978-1-4939-9940-8_10
Popis: In Parkinson's disease (PD) and related disorders pathological alpha-synuclein has been discussed to propagate via a prion-like mechanism in the CNS. The application of exogenous alpha-synuclein fibrils via injection to animal models of PD has been shown to be a useful method to study prion-like propagation of pathological alpha-synuclein and of transmission pathways that play a critical role in recapitulating characteristics of synucleinopathies. Using bigenic mice expressing mutant human alpha-synuclein in neurons and firefly luciferase in astrocytes we showed that transmission via the tongue and the peritoneum represent entrance points for pathological alpha-synuclein to invade the CNS. Here we present a method to quantify astrogliosis by bioluminescence imaging in an animal model of PD. This method allows noninvasive tracking of the neuroinflammatory process that often precedes neurological signs of disease and represents an alternative to behavioral or histological and biochemical analysis to detect disease.
Databáze: OpenAIRE
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