Cell fractionation analysis of human CD8 glycoprotein transport between endoplasmic reticulum, intermediate compartment and Golgi complex in tissue cultured cells
| Autor: | Luisa Iodice, Stefano Bonatti, Maria Carmen Erra, Lavinia Vittoria Lotti |
|---|---|
| Přispěvatelé: | Erra, M. C., Iodice, L., Lotti, L. V., Bonatti, Stefano |
| Rok vydání: | 1999 |
| Předmět: |
Glycosylation
CD8 Antigens Golgi Apparatus Cell Fractionation Endoplasmic Reticulum Transfection symbols.namesake Humans Cellular compartment Secretory pathway Cells Cultured Glycoproteins Glycoprotein transport Chemistry Vesicular-tubular cluster Endoplasmic reticulum Temperature STIM1 Biological Transport Cell Biology General Medicine COPI Golgi apparatus Cell biology Cell Compartmentation Microscopy Electron symbols |
| Popis: | We have set up an analytical cell fractionation procedure to dissect, by a non-morphological method, the anterograde transport of proteins from endoplasmic reticulum, intermediate compartment and Golgi complex in tissue cultured cells. Using this procedure after pulse-chase labelling of cells expressing human CD8 glycoprotein, we obtained results that: (1) support the view that the intermediate compartment is a distinct station in the export from the endoplasmic reticulum to the Golgi complex; and (2) strongly suggests that the O -glycosylation process starts after the intermediate compartment, presumably in the cis -Golgi complex. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|