Distinctive Microbial Signatures and Gut-Brain Crosstalk in Pediatric Patients with Coeliac Disease and Type 1 Diabetes Mellitus
| Autor: | Mamoun Elawad, Bara Al-Jarrah, Hoda Gad, Souhaila Al Khodor, Mohammed A. Hendaus, Khalid Hussain, Rayaz A. Malik, Anthony K Akobeng, Arun Rawat, Wesam Almasri, Saras Saraswathi, Parul Singh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pilot Projects Gut flora medicine.disease_cause Coeliac disease lcsh:Chemistry Cornea Pathogenesis 0302 clinical medicine T1DM Shigella Child lcsh:QH301-705.5 Spectroscopy biology Brain General Medicine Amplicon Computer Science Applications Trigeminal Nerve Diseases corneal confocal microscopy 030211 gastroenterology & hepatology Adolescent pediatric neuropathy Article Catalysis Inorganic Chemistry 03 medical and health sciences children Escherichia coli medicine Humans Physical and Theoretical Chemistry Molecular Biology Type 1 diabetes gut microbiota Bacteroidetes Organic Chemistry medicine.disease biology.organism_classification Gastrointestinal Microbiome Celiac Disease Cross-Sectional Studies Diabetes Mellitus Type 1 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Immunology Dysbiosis coeliac disease |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 4 International Journal of Molecular Sciences, Vol 22, Iss 1511, p 1511 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22041511 |
| Popis: | Coeliac disease (CD) and Type 1 diabetes mellitus (T1DM) are immune-mediated diseases. Emerging evidence suggests that dysbiosis in the gut microbiome plays a role in the pathogenesis of both diseases and may also be associated with the development of neuropathy. The primary goal in this cross-sectional pilot study was to identify whether there are distinct gut microbiota alterations in children with CD (n = 19), T1DM (n = 18) and both CD and T1DM (n = 9) compared to healthy controls (n = 12). Our second goal was to explore the relationship between neuropathy (corneal nerve fiber damage) and the gut microbiome composition. Microbiota composition was determined by 16S rRNA gene sequencing. Corneal confocal microscopy was used to determine nerve fiber damage. There was a significant difference in the overall microbial diversity between the four groups with healthy controls having a greater microbial diversity as compared to the patients. The abundance of pathogenic proteobacteria Shigella and E. coli were significantly higher in CD patients. Differential abundance analysis showed that several bacterial amplicon sequence variants (ASVs) distinguished CD from T1DM. The tissue transglutaminase antibody correlated significantly with a decrease in gut microbial diversity. Furthermore, the Bacteroidetes phylum, specifically the genus Parabacteroides was significantly correlated with corneal nerve fiber loss in the subjects with neuropathic damage belonging to the diseased groups. We conclude that disease-specific gut microbial features traceable down to the ASV level distinguish children with CD from T1DM and specific gut microbial signatures may be associated with small fiber neuropathy. Further research on the mechanisms linking altered microbial diversity with neuropathy are warranted. |
| Databáze: | OpenAIRE |
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