Evidence for Up-Regulation of Purinergic Receptor Genes Associating with TRPV1 Receptors and Neurotrophic Factors in the Inflamed Human Esophagus
| Autor: | K.-R. Shieh, H.-L. Tseng, C.-L. Chen, T.-T. Liu, C.-H. Yi, S.-C. Yang |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male TRPV4 medicine.medical_specialty Pathology Adolescent TRPV1 Gene Expression TRPV Cation Channels Biochemistry Young Adult Esophagus Neurotrophic factors Internal medicine Nerve Growth Factor Glial cell line-derived neurotrophic factor medicine Humans Glial Cell Line-Derived Neurotrophic Factor Receptor Esophagitis Peptic Molecular Biology Aged Aged 80 and over biology urogenital system musculoskeletal neural and ocular physiology Purinergic receptor General Medicine Middle Aged medicine.disease Up-Regulation Endocrinology Nerve growth factor nervous system Case-Control Studies biology.protein GERD Molecular Medicine Female Receptors Purinergic P2X7 Receptors Purinergic P2X3 |
| Zdroj: | Current Molecular Medicine. 14:1205-1214 |
| ISSN: | 1566-5240 |
| Popis: | Purinergic receptors are implicated in nociceptive signaling in small primary afferents via activation of adenosine triphosphate (ATP). ATP appears to mediate HCl-induced transient receptor potential vanilloid receptor 1 (TRPV1) activation in esophageal mucosa. Up-regulation of TRPV1 expression in gastroesophageal reflux disease (GERD) is associated with increased nerve growth factor (NGF) and glial derived neurotrophic factor (GDNF). This study aims to genetically determine the expression of purinergic receptors in severe inflamed human esophagus. Distal esophageal biopsies from the subjects with erosive GERD, asymptomatic patients (AP) and healthy ones were examined. Using real-time qPCR for detecting purinergic receptors (P2X 2 , P2X 3 , P2X 7 , P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 and P2Y 12 ), TRPV1, TRPV4, NGF, and GDNF was done in this study. Both P2X 3 and P2X 7 mRNA expressions in GERD patients significantly increased than those in healthy controls (P 2 , P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 or P2Y 12 had no difference within the control, AP or GERD subjects. The well correlated expression in P2X 3 gene with TRPV1 (r = 0.46, P = 0.002), NGF (r = 0.54, P = 0.0002), and GDNF (r = 0.64, P = 0.0001) was found. The P2X 7 gene expressions also well correlated with TRPV1 (r = 0.47, P = 0.002), NGF (r = 0.32, P = 0.037), and GDNF (r = 0.42, P = 0.005). These results suggest that chronic esophagitis increases mRNA expressions of P2X 3 and P2X 7 receptors accompanied by up-regulation of TRPV1 and neurotrophic factors (NGF and GDNF). These genetical alterations in esophageal mucosa might mediate sensitization of inflamed human esophagus. |
| Databáze: | OpenAIRE |
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