Coupling meiotic chromosome axis integrity to recombination
| Autor: | Denise Zickler, Silvana Gargano, Stefanie Pöggeler, Sophie Tessé, Aurora Storlazzi, Gwenaël Ruprich-Robert, Nancy Kleckner |
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| Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
MESH: Sordariales
Mitotic crossover MESH: Mutation Sordariales Fluorescent Antibody Technique Mitosis chromosome axes Biology Genetic recombination MESH: Chromatin Fungal Proteins 03 medical and health sciences 0302 clinical medicine Prophase Meiosis MESH: Plasmids Centromere Genetics Ectopic recombination [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Fluorescent Antibody Technique 030304 developmental biology Recombination Genetic 0303 health sciences Cohesin Rec8 Spo76/Pds5 MESH: Mitosis MESH: Chromosomes Fungal recombination Chromatin Cell biology MESH: Meiosis [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Mutation MESH: Fungal Proteins MESH: Recombination Genetic Chromosomes Fungal 030217 neurology & neurosurgery Plasmids Research Paper Developmental Biology |
| Zdroj: | Genes and Development Genes and Development, Cold Spring Harbor Laboratory Press, 2008, 22 (6), pp.796-809. ⟨10.1101/gad.459308⟩ Genes & development 22 (2008): 796–809. doi:10.1101/gad.459308 info:cnr-pdr/source/autori:Storlazzi A.; Tesse S.; Ruprich-Robert G.; Gargano S.; Poeggeler S.; Kleckner N.; Zickler D./titolo:Coupling meiotic chromosome axis integrity to recombination/doi:10.1101%2Fgad.459308/rivista:Genes & development/anno:2008/pagina_da:796/pagina_a:809/intervallo_pagine:796–809/volume:22 |
| ISSN: | 0890-9369 |
| DOI: | 10.1101/gad.459308⟩ |
| Popis: | International audience; During meiosis, DNA events of recombination occur in direct physical association with underlying chromosome axes. Meiotic cohesin Rec8 and cohesin-associated Spo76/Pds5 are prominent axis components. Two observations indicate that recombination complexes can direct the local destabilization of underlying chromosome axes. First, in the absence of Rec8, Spo76/Pds5 is lost locally at sites of late-persisting Msh4 foci, with a concomitant tendency for loosening of intersister and interhomolog connectedness at the affected sites. This loss is dependent on initiation of recombination. Second, in wild-type prophase, local separation of sister axes is seen at sites of synaptonemal complex-associated recombination nodules. Additional findings reveal that Rec8 localizes to both axis and bulk chromatin and is required for chromatin compactness. Further, Rec8 is essential for maintenance of sister cohesion, along arms and centromeres, during the pachytene-to-diplotene transition, revealing an intrinsic tendency for destabilization of sister cohesion during this period. This finding shows how the loss of sister connectedness, in arm and/or centric regions, could lead to the segregation defects that are seen in the human "maternal age effect" and how Rec8 could be a target of that effect. Finally, Rec8 plays related, but synergistic roles with Spo76/Pds5, indicating auxiliary roles for meiotic and mitotic cohesion-associated components. |
| Databáze: | OpenAIRE |
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