Mycobacterium tuberculosisexposure of livestock in a German dairy farm: implications forintra vitamdiagnosis of bovine tuberculosis in an officially tuberculosis-free country
| Autor: | Christian Menge, K. Riße, K. Schlez, Tobias Eisenberg, Irmgard Moser, N. Fiege, G. Kraft, H.P. Hamann, M. Zschöck, C. Sauerwald, A. Fawzy, G. Althoff, R. Riße, Ute Kaim, Anne Nesseler, U. Kling |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Veterinary medicine Tuberculosis Epidemiology 030106 microbiology Polymerase Chain Reaction law.invention Mycobacterium tuberculosis 03 medical and health sciences law Germany medicine Bovine tuberculosis Animals Polymerase chain reaction Mycobacterium bovis biology business.industry biology.organism_classification medicine.disease Infectious Diseases Herd Caseous lymphadenitis Cattle Female Livestock business Tuberculosis Bovine |
| Zdroj: | Epidemiology and Infection. 144:724-731 |
| ISSN: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268815001995 |
| Popis: | SUMMARYGermany has been an officially bovine tuberculosis (bTB)-free (OTF) country since 1996. Gradually rising numbers of bTB herd incidents due toMycobacterium bovisandM. capraein North-Western and Southern Germany during the last few years prompted the competent authorities to conduct a nationwide bTB survey in 2013/2014. This led to the detection of a dairy herd in which as many as 55 cattle reacted positively to consecutiveintra vitamtesting. Test-positive animals lacked visible lesions indicative of bTB at necropsy. Extensive mycobacterial culturing as well as molecular testing of samples from 11 tissues for members of theM. tuberculosiscomplex (MTC) yielded negative results throughout. However, caseous lymphadenitis of Ln. mandibularis accessorius was observed during meat inspection of a fattening pig from the same farm at regular slaughter at that time. Respective tissue samples tested MTC positive by polymerase chain reaction, andM. tuberculosisT1 family were identified by spoligotyping. Four human reactors within the farmer's family were also found to be immunoreactive. As exposure of livestock toM. tuberculosisis not generally considered, its impact may result in regulatory and practical difficulties when using protocols designed to detect classical bTB, particularly in OTF countries. |
| Databáze: | OpenAIRE |
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