Real-time metabolic imaging
| Autor: | Mikkel Thaning, René in ‘t Zandt, Klaes Golman |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Alanine Magnetic Resonance Spectroscopy Time Factors Multidisciplinary Cell Survival Swine Chemistry Endogeny Metabolism Magnetic Resonance Imaging Models Biological Energy homeostasis Rats chemistry.chemical_compound Biochemistry Physical Sciences Pyruvic Acid Image Processing Computer-Assisted Animals Viability assay Hyperpolarization (physics) Pyruvic acid Rats Wistar Molecular imaging |
| Zdroj: | Proceedings of the National Academy of Sciences. 103:11270-11275 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The endogenous substance pyruvate is of major importance to maintain energy homeostasis in the cells and provides a window to several important metabolic processes essential to cell survival. Cell viability is therefore reflected in the metabolism of pyruvate. NMR spectroscopy has until now been the only noninvasive method to gain insight into the fate of pyruvate in the body, but the low NMR sensitivity even at high field strength has only allowed information about steady-state conditions. The medically relevant information about the distribution, localization, and metabolic rate of the substance during the first minute after the injection has not been obtainable. Use of a hyperpolarization technique has enabled 10–15% polarization of 13 C 1 in up to a 0.3 M pyruvate solution. i.v. injection of the solution into rats and pigs allows imaging of the distribution of pyruvate and mapping of its major metabolites lactate and alanine within a time frame of ≈10 s. Real-time molecular imaging with MRI has become a reality. |
| Databáze: | OpenAIRE |
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