Comparative expression analysis of heavy metal ATPase subfamily genes between Cd-tolerant and Cd-sensitive turnip landraces
| Autor: | Yonghong Yang, Yuansheng Wu, Xi Han, Di Chen, Yongping Yang, Xiong Li, Boqun Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Subfamily pI isoelectric point Plant Science Biology SSI seedling survival index 010603 evolutionary biology 01 natural sciences Genome Subclass Article MW molecular weight Phylogenetics lcsh:Botany Coding region Turnip HMA heavy metal ATPase lcsh:QH301-705.5 Gene Differential gene expression Ecology Evolution Behavior and Systematics Heavy metal ATPase Genetics HM heavy metal Phylogenetic tree Chromosome food and beverages GPI germination percentage index lcsh:QK1-989 CK control blank GRAVY grand average of hydropathicity lcsh:Biology (General) Transmembrane helix 010606 plant biology & botany Cadmium |
| Zdroj: | Plant Diversity Plant Diversity, Vol 41, Iss 4, Pp 275-283 (2019) |
| ISSN: | 2468-2659 |
| Popis: | The heavy metal ATPase (HMA) subfamily is mainly involved in heavy metal (HM) tolerance and transport in plants, but an understanding of the definite roles and mechanisms of most HMA members are still limited. In the present study, we identified 14 candidate HMA genes named BrrHMA1–BrrHMA8 from the turnip genome and analyzed the phylogeny, gene structure, chromosome distribution, and conserved domains and motifs of HMAs in turnip (Brassica rapa var. rapa). According to our phylogenetic tree, the BrrHMAs are divided into a Zn/Cd/Co/Pb subclass and Cu/Ag subclass. The BrrHMA members show similar structural characteristics within subclasses. To explore the roles of BrrHMAs in turnip, we compared the gene sequences and expression patterns of the BrrHMA genes between a Cd-tolerant landrace and a Cd-sensitive landrace. Most BrrHMA genes showed similar spatial expression patterns in both Cd-tolerant and Cd-sensitive turnip landraces; some BrrHMA genes, however, were differentially expressed in specific tissue in Cd-tolerant and Cd-sensitive turnip. Specifically, BrrHMA genes in the Zn/Cd/Co/Pb subclass shared the same coding sequence but were differentially expressed in Cd-tolerant and Cd-sensitive turnip landraces under Cd stress. Our findings suggest that the stable expression and up-regulated expression of BrrHMA Zn/Cd/Co/Pb subclass genes under Cd stress may contribute to the higher Cd tolerance of turnip landraces. Keywords: Heavy metal ATPase, Cadmium, Turnip, Transmembrane helix, Differential gene expression |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|