Two Validated HPLC Methods for the Quantification of Alizarin and other Anthraquinones in Rubia tinctorum Cultivars
| Autor: | Goverdina C. H. Derksen, T.A. van Beek, G.P. Lelyveld, Anthony Capelle, Ae. de Groot |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Rubia tinctorum
growth Anthraquinones madder root Plant Science Alizarin morinda-citrifolia Plant Roots Biochemistry High-performance liquid chromatography Anthraquinone Analytical Chemistry quantitative-analysis chemistry.chemical_compound Drug Discovery hydroxyanthraquinones Cultivar Chromatography High Pressure Liquid chemistry.chemical_classification performance liquid-chromatography Chromatography Molecular Structure biology EPS-3 Rubia Organic Chemistry Glycoside General Medicine biology.organism_classification Organische Chemie lucidin tissue-cultures Complementary and alternative medicine chemistry Molecular Medicine identification Ruberythric acid Food Science cell-suspension cultures |
| Zdroj: | Phytochemical Analysis 15 (2004) 6 Phytochemical Analysis, 15(6), 397-406 |
| ISSN: | 0958-0344 |
| Popis: | Direct and indirect HPLC-UV methods for the quantitative determination of anthraquinones in dried madder root have been developed, validated and compared. In the direct method, madder root was extracted twice with refluxing ethanol–water. This method allowed the determination of the two major native anthraquinone glycosides lucidin primeveroside and ruberythric acid. In the indirect extraction method, the anthraquinone glycosides were first converted into aglycones by endogenous enzymes and the aglycones were subsequently extracted with tetrahydrofuran–water and then analysed. In this case the anthraquinones alizarin, purpurin and nordamnacanthal may be determined. The content of nordamnacanthal is proportional to the amount of lucidin primeveroside originally present. The indirect extraction method is easier to apply. Different madder cultivars were screened for their anthraquinone content. Copyright © 2004 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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