Identification and characterization of ANO9 in stage II and III colorectal carcinoma

Autor: Sanjun Cai, Zheng Jiang, Xishan Wang, Chunxiang Li
Rok vydání: 2015
Předmět:
Male
Oncology
Pathology
Time Factors
Colorectal cancer
Apoptosis
Kaplan-Meier Estimate
medicine.disease_cause
Metastasis
Cell Movement
Risk Factors
Phospholipid Transfer Proteins
Aged
80 and over
Middle Aged
Flow Cytometry
Immunohistochemistry
Up-Regulation
ANO9
Gene Expression Regulation
Neoplastic
Treatment Outcome
Real-time polymerase chain reaction
Female
RNA Interference
Colorectal Neoplasms
Research Paper
Signal Transduction
medicine.medical_specialty
Anoctamins
colorectal cancer
Real-Time Polymerase Chain Reaction
Transfection
Internal medicine
Biomarkers
Tumor
medicine
Carcinoma
metastasis
Humans
Neoplasm Invasiveness
Clinical significance
RNA
Messenger
Aged
Cell Proliferation
Proportional Hazards Models
business.industry
Membrane Proteins
Cancer
HCT116 Cells
medicine.disease
prognosis
Caco-2 Cells
Neoplasm Recurrence
Local
Carcinogenesis
business
Zdroj: Oncotarget
ISSN: 1949-2553
Popis: // Chunxiang Li 1 , Sanjun Cai 2 , Xishan Wang 3,4 and Zheng Jiang 3,4 1 Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang, China 2 Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China 3 Department of Colorectal Cancer Surgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China 4 Colorectal Cancer Institute of the Heilongjiang Academy of Medical Sciences, Harbin, Heilongjiang, China Correspondence to: Zheng Jiang, email: // Keywords : ANO9, colorectal cancer, prognosis, metastasis Received : May 03, 2015 Accepted : July 11, 2015 Published : July 22, 2015 Abstract Background and Objectives: The precise role and potential underlying mechanisms of anoctamin 9 ( ANO9 ) remain largely unknown. This study aims to characterize the role and oncogenic mechanisms of ANO9 in stage II and III colorectal cancer (CRC). Methods: We examined the expression of ANO9 in colorectal cancerous tissues and cells using real-time quantitative PCR and immunohistochemistry, respectively. Multiple cellular and molecular approaches such as gene transfection, CCK-8 assay, flow cytometry, and invasion assay were also performed to explore its oncogenic mechanisms. Furthermore, the clinical significance of ANO9 in clinical CRC specimens was assessed by clinical correlation and survival analyses. Results: Lower expression of ANO9 messenger RNA (mRNA) was frequently detected both in CRC tissues with recurrence and metastasis-derived cell lines. Compared with matched nontumorous tissues, lower expression of ANO9 protein was observed in tumors, which was significantly correlated with tumorigenesis ( p < 0.05). In vitro functional studies showed that AN O9 contributed to tumor cell proliferation, apoptosis, and invasion. Moreover, investigation of clinical CRC specimens showed that ANO9 were markedly overexpressed in metastatic tissue compared with primary tissue. Decreased expression of ANO9 was correlated with poor prognostic outcomes. Conclusions: This study highlighted the role of ANO9 in progression and metastasis of stage II and III CRC. These findings suggested that up-regulation of ANO9 , as a metastasis-related gene, could be a novel approach for inhibiting CRC progression.
Databáze: OpenAIRE
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