Preparation of novel anti-ski monoclonal antibodies
Autor: | Jian-Bin Tong, Wei-Jun Cai, Feng Ye, Da-Zhi Zhang, Yixuan Yang, Sang-Lin Li, Hong Ren, Peng Hu, Huaidong Hu |
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Rok vydání: | 2011 |
Předmět: |
animal structures
medicine.drug_class Recombinant Fusion Proteins Immunology Antibody Affinity Spleen SMAD Immunoglobulin light chain Monoclonal antibody Avian sarcoma virus Subclass Mice Western blot Proto-Oncogene Proteins medicine Escherichia coli Immunology and Allergy Animals Humans Cloning Molecular Mice Inbred BALB C biology medicine.diagnostic_test Antibodies Monoclonal Virology Molecular biology Peptide Fragments DNA-Binding Proteins medicine.anatomical_structure Gene Expression Regulation Immunoglobulin G biology.protein Antibody human activities |
Zdroj: | Hybridoma (2005). 30(4) |
ISSN: | 1557-8348 |
Popis: | Ski is an avian sarcoma virus oncogene homolog best known for inhibiting TGF beta signaling through its association with the SMAD proteins. Anti-Ski antibodies (MAbs) of high titer were prepared by immunizing BALB/c mice with multifocal intradermal injections and fusing high titer antibody producing spleen cells with myeloma cells of SP2/0 origin. Three MAbs were selected for further characterization as classes and subclasses. Antibodies were produced by these three clones with high affinities ranging from 10(9) to 10(11)/m. These clones were found to be of the immunoglobulin IgG1 and IgG2b subclass with kappa light chain. They could recognize Ski as determined by Western blot analysis. The produced MAbs will be a useful tool for further investigation of Ski functions in organisms. |
Databáze: | OpenAIRE |
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