Glutamine, Glutamate, and α-Glucosylglycerate Are the Major Osmotic Solutes Accumulated by Erwinia chrysanthemi Strain 3937
| Autor: | Carlos Blanco, Renan Goude, Sylvie Bonnassie, Stéphanie Renaud, Théophile Bernard |
|---|---|
| Přispěvatelé: | Interactions cellulaires et moléculaires (ICM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2004 |
| Předmět: |
Glutamine
Pectobacterium chrysanthemi Biology Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound MESH: Glutamates MESH: Osmolar Concentration Betaine Glutamates Osmotic Pressure MESH: Glycolipids 030304 developmental biology MESH: Glutamine 0303 health sciences Growth medium Ecology Osmotic concentration 030306 microbiology Osmolar Concentration Dickeya chrysanthemi Water-Electrolyte Balance MESH: Osmotic Pressure Physiology and Biotechnology MESH: Pectobacterium chrysanthemi Trehalose Culture Media [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Biochemistry Osmolyte MESH: Betaine MESH: Culture Media MESH: Water-Electrolyte Balance bacteria Osmoprotectant Glycolipids Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2004, 70 (11), pp.6535-41. ⟨10.1128/AEM.70.11.6535-6541.2004⟩ Applied and Environmental Microbiology, 2004, 70 (11), pp.6535-41. ⟨10.1128/AEM.70.11.6535-6541.2004⟩ |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.70.11.6535-6541.2004 |
| Popis: | Erwinia chrysanthemi is a phytopathogenic soil enterobacterium closely related to Escherichia coli . Both species respond to hyperosmotic pressure and to external added osmoprotectants in a similar way. Unexpectedly, the pools of endogenous osmolytes show different compositions. Instead of the commonly accumulated glutamate and trehalose, E. chrysanthemi strain 3937 promotes the accumulation of glutamine and α-glucosylglycerate, which is a new osmolyte for enterobacteria, together with glutamine. The amounts of the three osmolytes increased with medium osmolarity and were reduced when betaine was provided in the growth medium. Both glutamine and glutamate showed a high rate of turnover, whereas glucosylglycerate stayed stable. In addition, the balance between the osmolytes depended on the osmolality of the medium. Glucosylglycerate and glutamate were the major intracellular compounds in low salt concentrations, whereas glutamine predominated at higher concentrations. Interestingly, the ammonium content of the medium also influenced the pool of osmolytes. During bacterial growth with 1 mM ammonium in stressing conditions, more glucosylglycerate accumulated by far than the other organic solutes. Glucosylglycerate synthesis has been described in some halophilic archaea and bacteria but not as a dominant osmolyte, and its role as an osmolyte in Erwinia chrysanthemi 3937 shows that nonhalophilic bacteria can also use ionic osmolytes. |
| Databáze: | OpenAIRE |
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