Sperm Wash in Three Culture Media: Maximization of Motile Sperm Recovery During Swim-Up Incubation
| Autor: | R Vijayakumar, W. Heine, F De Leon, B. Ndubisi |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system Urology medicine.medical_treatment Motility In Vitro Techniques Biology Andrology Endocrinology medicine Humans Incubation reproductive and urinary physiology Sperm motility urogenital system Artificial insemination Sperm washing Motile sperm General Medicine Spermatozoa Sperm Culture Media Kinetics medicine.anatomical_structure Sperm Motility Gamete |
| Zdroj: | Andrologia. 19:579-584 |
| ISSN: | 1439-0272 0303-4569 |
| Popis: | Three different culture media commonly used during in vitro gamete manipulations were studied for their efficacy in sperm wash procedure. Highest numbers of motile sperm were recovered at 6 hours following incubation in WT-6 and Ham's F-10 media. However, WT-6 yielded higher motile sperm numbers than Ham's F-10. Swim-up sperm number reached a peak at 3 hours following incubation in BWW. A period of 2 to 6 hours of incubation of sperm pellets overlayed with sperm wash media resulted in highly enriched motile sperm fractions free of dead spermatozoa and seminal debri. |
| Databáze: | OpenAIRE |
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