Differences in bacterial composition between men's and women's restrooms and other common areas within a public building
| Autor: | Álvaro Macedo Laureano, Geferson Fernando Metz, Filipe de Carvalho Victoria, Marc Redmile-Gordon, Anderson Santos de Freitas, André Carlos Cruz Copetti, Priscila Caroline Thiago Dobbler, Ehidy Rocio Peña Cañón, Luiz Fernando Wurdig Roesch, Daniel Kumazawa Morais, Deise Schroder Sarzi, Cristiane Barbosa D Oliveira, Victor Satler Pylro, Beatriz Midori Takagaki |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Universities Range (biology) Firmicutes Beta diversity Microbiology Actinobacteria 03 medical and health sciences Microbial ecology RNA Ribosomal 16S Environmental Microbiology Humans Microbiome Molecular Biology biology Bacteria Ecology Microbiota Bacteroidetes Dust General Medicine Biodiversity biology.organism_classification Environment Controlled 030104 developmental biology Geography Female Proteobacteria Brazil Environmental Monitoring |
| Zdroj: | Antonie van Leeuwenhoek. 111(4) |
| ISSN: | 1572-9699 |
| Popis: | Humans distribute a wide range of microorganisms around building interiors, and some of these are potentially pathogenic. Recent research established that humans are the main drivers of the indoor microbiome and up to now significant literature has been produced about this topic. Here we analyzed differences in bacterial composition between men's and women's restrooms and other common areas within the same public building. Bacterial DNA samples were collected from restrooms and halls of a three-floor building from the Federal University of Pampa, RS, Brazil. The bacterial community was characterized by amplification of the V4 region of the 16S rRNA gene and sequencing. Throughout all samples, the most abundant phylum was Proteobacteria, followed by Actinobacteria, Bacteroidetes and Firmicutes. Beta diversity metrics showed that the structure of the bacterial communities were different among the areas and floors tested, however, only 6-9% of the variation in bacterial communities was explained by the area and floors sampled. A few microorganisms showed significantly differential abundance between men's and women's restrooms, but in general, the bacterial communities from both places were very similar. Finally, significant differences among the microbial community profile from different floors were reported, suggesting that the type of use and occupant demographic within the building may directly influence bacterial dispersion and establishment. |
| Databáze: | OpenAIRE |
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