Unexpected elution behaviour of peptides with various reversed-phase columns

Autor:	Heinz Engelhardt, E. Schweinheim, G. Appelt
Rok vydání:	1990
Předmět:	chemistry.chemical_classification Chromatography Chemistry Elution Organic Chemistry Peptide General Medicine Reversed-phase chromatography Biochemistry Analytical Chemistry Chain length Phase (matter) Gastrins Polar Cholecystokinin Peptides Chromatography High Pressure Liquid Gastrin
Zdroj:	Journal of chromatography. 499
Popis:	Gastrin and cholecystokinin peptides were separated by reversed-phase chromatography on conventional bristle-type and polymer-coated stationary phases. The retention of the sulphated and non-sulphated isomeric forms of both peptides is governed by the structure of the peptide, the net charge and additional polar interactions with the stationary phases. Polymer-coated phases are optimum for separations according to chain length, whereas polar interactions are required for the separation of sulphated and non-sulphated peptides of identical chain length.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::293e8e7d125c412ba37719077647284c https://pubmed.ncbi.nlm.nih.gov/2324206 Zobrazit plný text záznamu