Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase
| Autor: | Marco Terreni, Roberto Xaiz, Simona Daly, Davide A. Cecchini, Massimo Pregnolato, Sara Sanna, Roberto Pavesi |
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| Rok vydání: | 2012 |
| Předmět: |
Immobilized enzyme
Protein Engineering Applied Microbiology and Biotechnology Industrial Microbiology Hydrolysis chemistry.chemical_compound Cefprozil Enzyme Stability Escherichia coli Amidase activity Site-directed mutagenesis Molecular Structure Escherichia coli Proteins Rational design General Medicine Enzymes Immobilized Combinatorial chemistry Anti-Bacterial Agents Cephalosporins Kinetics Biochemistry chemistry Biocatalysis Yield (chemistry) Mutagenesis Site-Directed Penicillin Amidase Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 95:1491-1500 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | We describe the rational design of a new efficient biocatalyst and the development of a sustainable green process for the synthesis of cephalosporins bearing a NH₂ group on the acyl side chain. The new biocatalyst was developed starting from the WT penicillin acylase (PA) from Escherichia coli by combining enzyme mutagenesis, in position α146 and β24 (βF24A/αF146Y), and immobilization on an appropriate modified industrial support, glyoxyl Eupergit C250L. The obtained derivative was used in the kinetically controlled synthesis of cephalexin, cefprozil and cefaclor and compared to the WT-PA and an already described mutant, PA-βF24A, with improved properties. The new biocatalyst posses a very high ratio between the rates of the synthesis and two undesired hydrolyses (acylating ester and the amidic product). In particular, a very low amidase activity was observed with PA-βF24A/αF146Y and, consequently, the hydrolysis of the produced antibiotic was avoided during the process. Taking advantage of this property, higher conversions in the synthesis of cephalexin (99% versus 76%), cefaclor (99% versus 65%) and cefprozil (99% versus 60%) were obtained compared to the WT enzyme. Furthermore, the new mutant also show a higher synthetic activity compared to PA-βF24A immobilized on the same support, allowing the maximum yields to be achieved in very short reaction times. The production of cephalexin with the immobilized βF24A/αF146Y acylase has been developed on a pre-industrial scale (30 l). After 20 cycles, the average yield was 93%. The biocatalyst showed good stability properties and no significant decrease in performance. |
| Databáze: | OpenAIRE |
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