Progenitor/Stem Cell Markers in Brain Adjacent to Glioblastoma: GD3 Ganglioside and NG2 Proteoglycan Expression

Autor: Lama, Gina, Mangiola, Annunziato, Proietti, Gabriella, Colabianchi, Anna, Angelucci, Cristiana, D'Alessio, A, De Bonis, Pasquale, Geloso, Maria Concetta, Lauriola, Libero, Binda, E, Biamonte, Filippo, Giuffrida, Mg, Vescovi, A, Sica, Gigliola, D'Alessio, Alessio
Přispěvatelé: Lama, G, Mangiola, A, Proietti, G, Colabianchi, A, Angelucci, C, D'Alessio, A, De Bonis, P, Geloso, M, Lauriola, L, Binda, E, Biamonte, F, Giuffrida, M, Vescovi, A, Sica, G
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Pathology
Mice
SCID
NG2 proteoglycan
Stem cell marker
Nestin
angiogenesis
Mice
0302 clinical medicine
Gangliosides
GD3 ganglioside
Brain adjacent to tumor
Tumor
biology
Brain Neoplasms
Sialyltransferase
Stem Cells
General Medicine
Middle Aged
Immunohistochemistry
Neurology
Antigen
030220 oncology & carcinogenesis
lipids (amino acids
peptides
and proteins)
Female
Proteoglycans
Settore BIO/17 - ISTOLOGIA
Stem cell
Human
Adult
medicine.medical_specialty
glioblastoma
Brain adjacent to tumor
Cancer stem cells
GD3 ganglioside
NG2 proteoglycan
SCID
Pathology and Forensic Medicine
NO
Brain Neoplasm
03 medical and health sciences
Cellular and Molecular Neuroscience
Young Adult
Cancer stem cell
Biomarkers
Tumor
medicine
Animals
Humans
Actins
Aged
Antigens
Brain Chemistry
Glioblastoma
Karnofsky Performance Status
Sialyltransferases
Actin
Progenitor
proteoglycan
Settore MED/08 - ANATOMIA PATOLOGICA
Animal
stem cell
030104 developmental biology
Proteoglycan
nervous system
Ganglioside
biology.protein
Karnofsky Performance Statu
Neurology (clinical)
Biomarkers
Zdroj: Europe PubMed Central
Popis: Characterization of tissue surrounding glioblastoma (GBM) is a focus for translational research because tumor recurrence invariably occurs in this area. We investigated the expression of the progenitor/ stem cell markers GD3 ganglioside and NG2 proteoglycan in GBM, peritumor tissue (brain adjacent to tumor, BAT) and cancer stem-like cells (CSCs) isolated from GBM (GCSCs) and BAT (PCSCs). GD3 and NG2 immunohistochemistry was performed in paired GBM and BAT specimens from 40 patients. Double-immunofluorescence was carried out to characterize NG2-positive cells of vessel walls. GD3 and NG2 expression was investigated in GCSCs and PCSCs whose tumorigenicity was also evaluated in Scid/bg mice. GD3 and NG2 expression was higher in tumor tissue than in BAT. NG2 decreased as the distance from tumor margin increased, regardless of the tumor cell presence, whereas GD3 correlated with neoplastic infiltration. In BAT, NG2 was coexpressed with a-smooth muscle actin (α-SMA) in pericytes and with nestin in the endothelium. Higher levels of NG2 mRNA and protein were found in GCSCs while GD3 synthase was expressed at similar levels in the 2 CSC populations. PCSCs had lower tumorigenicity than GCSCs. These data suggest the possible involvement of GD3 and NG2 in pre/pro-tumorigenic events occurring in the complex microenvironment of the tissue surrounding GBM.
Databáze: OpenAIRE
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