Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment
| Autor: | Yanni Song, Lihua Fang, Yonghui Lai, Fang Li, Hao Yi, Xiaodong Fang, Yinping Tian, Ying-Yan Zhou, Zhenhua Zeng, Yile Chen, Xiao-Hong He, Yi-Ting He, Jie Li, Chu Zhou, Pan Feng, Run-Yue Huang, Qiang Gao, Dongni Qiu, Jinqun Huang, Jiehua Lin, Yong-Yue Xu, Mingming Shi, Xi Tan |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
0301 basic medicine Microbiology (medical) Adolescent Heparan sulfate degradation Decoction Pathogenesis Gut flora medicine.disease_cause Microbiology Bacteroides nordii Young Adult 03 medical and health sciences 0302 clinical medicine medicine Humans Spondylitis Ankylosing Feces 030203 arthritis & rheumatology Ankylosing spondylitis biology business.industry Flavonifractor plautii General Medicine Middle Aged biology.organism_classification medicine.disease Gastrointestinal Microbiome Drug effect Clinical Microbiology 030104 developmental biology Immunology Celecoxib Parabacteroides distasonis Dysbiosis Metagenome business Research Article Drugs Chinese Herbal medicine.drug |
| Zdroj: | Journal of Medical Microbiology |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.001107 |
| Popis: | Introduction. Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome. Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy. Aim. We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions. In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients. Methodology. Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen–Qiangdu–Zhilv decoction, were collected and used in the drug effect analysis. All samples were sequenced using Illumina HiSeq 4000 and the microbial composition was determined. Results. Four species were enriched in the patients with AS: Flavonifractor plautii , Oscillibacter , Parabacteroides distasonis and Bacteroides nordii (HC vs. NM, P F. plautii was found to be significantly changed in the NM-HC comparison. No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains. F. plautii was found to be significantly increased in the comparison between the HC and WM groups, along with four other species ( Clostridium bolteae , Clostridiales bacterium 1_7_47FAA, C. asparagiforme and C. hathewayi ). The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces. They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins. Conclusion. The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence. Our data provide insight that could guide improvements in AS treatment. |
| Databáze: | OpenAIRE |
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