| Popis: |
Additional file 1: Table S1. Composition of empty fruit bunch (EFB) solution used as the carbon source. Figure S1. Time profiles of carbon utilization of recombinant C. glutamicum strains H36GD13032 (○, ○), H36GD1447 (△, △), and H36GD1852 (□, □) using different combinations of carbon sources. Glucose consumption is indicated as green lines and xylose is represented as blue lines (A: 50 g/L glucose, B: 40 g/L glucose, 10 g/L xylose, C: 30 g/L glucose and 20 g/L xylose, D: 20 g/L glucose and 30 g/L xylose). Figure S2. Gamma-aminobutyrate production by recombinant C. glutamicum strains H30GD13032, H30GD1447, and H30GD1852 after 120 h of flask cultivation in medium containing different combinations of carbon sources (A, 50 g/L glucose; B, 40 g/L glucose and 10 g/L xylose; C, 30 g/L glucose and 20 g/L xylose; D, 20 g/L glucose and 30 g/L xylose). Figure S3. Concentrations of xylose, glutamate and gamma-aminobutyrate after 120 h of flask cultivation using recombinant C. glutamicum H36GM1852 (gray) and C. glutamicum H36GD1852 (white). The culture medium used contained different combinations of carbon sources (50G, 50 g/L glucose; 40G10X, 40 g/L glucose and 10 g/L xylose; 30G20X, 30 g/L glucose and 20 g/L xylose; 20G30X, 20 g/L glucose and 30 g/L xylose). Figure S4. Gamma-aminobutyrate production by recombinant C. glutamicum H36GD1852 after 120 h of flask cultivation in medium containing different combinations of carbon sources (50G, 50 g/L glucose; 20G, 20 g/L glucose; 20G5X, 20 g/L glucose and 50 g/L xylose; 20G10X, 20 g/L glucose and 10 g/L xylose; 20G20X, 20 g/L glucose and 20 g/L xylose; 20G3X, 20 g/L glucose and 30 g/L xylose). Figure S5. Gamma-aminobutyrate production and glutamate accumulation by recombinant C. glutamicum H36GD1852 after 120 h of flask cultivation in medium containing 30:20 glucose to xylose ratio. Additional concentrations of PLP (0.1–0.4mM) was supplemented during cultivation. |
