Preparation of high-quality iodine-125-labelled pituitary luteinizing hormone for radioimmunoassay
| Autor: | Iracelia Torres de Toledo e Souza, Bernardo Léo Wajchenberg, Pieroni Rr, Heidi Pinto, Olga Zazuco Higa, Rubens Schmidt Werner |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
medicine.medical_specialty
Chromatography Paper Electrophoresis Starch Gel Clinical Biochemistry Radioimmunoassay Pituitary luteinizing hormone chemistry.chemical_element Iodine Biochemistry Iodine Radioisotopes Internal medicine Methods medicine Animals Incubation Biochemistry (medical) food and beverages General Medicine Luteinizing Hormone Electrophoresis Starch gel electrophoresis Endocrinology chemistry Talc Sephadex Chromatography Gel Rabbits Luteinizing hormone |
| Zdroj: | Clinica Chimica Acta. 60:125-135 |
| ISSN: | 0009-8981 |
| Popis: | High quality pituitary luteinizing hormone labelled with 125 I was obtained after separating out the more heavily iodinated fractions, through starch gel electrophoresis, using the cathodal component (Fraction 1) which was further purified on Sephadex G-100, giving an almost pure 1 2 5 I-labelled luteinizing hormone preparation, presenting excellent immunoreactivity and low levels of damage on incubation in plasma. The quality control of the steps of the technique was done with plasma-coated talc (200 mg) which compared favorably, as far as being an indication of intact labelled luteinizing hormone, with the more time-consuming chromato electrophoresis. |
| Databáze: | OpenAIRE |
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