Fluorescent characterization of amyloid deposits in the kidneys of mdx mice
| Autor: | Aleksandra Gudkova, Mikhail M. Shavlovsky, E. V. Mikhailova, Dmitry Korzhevskii, V. V. Gusel’nikova, O. I. Antimonova, Aleksandr Krutikov, E. A. Fedorova, Vyacheslav Mikhailov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
Pathology medicine.medical_specialty Histology Amyloid Biophysics Plaque Amyloid Kidney Renal amyloidosis Fluorescence Congo red 03 medical and health sciences chemistry.chemical_compound Mice fluorene 0302 clinical medicine mental disorders medicine Animals lcsh:QH301-705.5 Fixative Fluorescent Dyes amyloidosis Original Paper Amyloidosis 7-(1-amino-4-sulfo-2- naphthylazo) fluorene (DSNAF) Histological Techniques Cell Biology medicine.disease Staining chemistry lcsh:Biology (General) 030220 oncology & carcinogenesis Mice Inbred mdx Thioflavin T Thioflavin Female Kidney Diseases mdx mice 030217 neurology & neurosurgery |
| Zdroj: | European Journal of Histochemistry, Vol 62, Iss 2 (2018) European Journal of Histochemistry : EJH |
| ISSN: | 2038-8306 |
| Popis: | Amyloidosis is a group of diseases that occurs when amyloid proteins are deposited in tissues and organs. The traditional way of identifying amyloid in tissue sections is staining with Congo red. However, this method has a number of limitations including background staining (background fluorescence), low fluorescence intensity and false-positive staining. Therefore, a complex of fluorescence-based methods should be applied to characterize tissue localization of amyloid deposits. The aim of this study was to identify amyloid deposits in the kidneys of dystrophin-deficient mdx mice using different fluorescent dyes. We examined 8 cases of renal amyloidosis in aged mdx mice. In all cases, we used traditional methods for amyloid detection (Congo red and Thioflavin T), as well as a new fluorescent dye, disodium salt of 2,7- (1-amino-4-sulfo-2-naphthylazo) fluorene (DSNAF). In our study, we confirmed the amyloid structure of protein deposits in kidneys of aging mdx mice by several fluorescence-based staining methods. We found that fixation method has profound effects on downstream staining procedures, and demonstrated that the application of specific fixative, zinc-ethanol-formaldehyde (ZEF), instead of traditional NBF allow to reduce the background fluorescence. We also illustrated the usefulness of novel fluorescent dye DSNAF for detection of amyloid deposits in mouse tissues. Our results confirmed the strong affinity and high specificity of this dye for amyloid fibrils. The verification of DSNAF for detecting amyloid in human tissues will provide a conclusion on the applicability of the developed staining method in clinical research practice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|